Effects of Browning Inhibitors on Callus Growth and Secondary Metabolites Production in Tripterygium wilfordii Hook f.

To study the effects of browning inhibitors on callus growth and secondary metabolites content,the callus of Tripterygium wilfordii root and the NT were used as basic medium,supplemented with different concentrations of Na2S2O3,Vc,citric acid,AgNO3,PV,and active carbon.The results showed that callus growth in T.wilfordii was inhibited in all treatments,in addition to the citric acid with a lower concentration.Although the browning could not be controlled when the medium was supplemented with Na2S2O3,Vc,Citric acid，AgNO3，PV,and active carbon,the triptolide content increased significantly in all treatments,improving up to 2.4 times with the addition of 50 mg/L citric acid.However,the addition of 10-50 mg/LAgNO3 in the medium,inhibited callus browning and increased the triptolide content with the increase in AgNO3 concentration.Alkaloids content were inhibited by using other browning inhibitors,in addition to the Na2S2O3 with a lower concentration.