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XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
Citation: XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
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Research Progress on Plant Expression Vectors for Gateway Cloning Technology

  • Biotechnology Research Centre, Kunming University of Science and Technology, Kunming 650500, China

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  • Received Date: March 01, 2012
  • Revised Date: July 10, 2012
  • Published Date: October 29, 2012
    Graphical Abstract
    • Abstract
  • Gateway molecular cloning technology was developed by Invitrogen based on the mechanism of site-specific recombination between λ phage DNA and the Escherichia coli genome.Construction of an expression vector for a target gene via Gateway LR reaction does not need to use restriction enzymes and a ligase,and thus is time efficient. Recently,to expand the application of Gateway technology in plant genetic engineering,several research groups have constructed Gateway destination vectors for studies on constitutive,inducible or silence expression of target genes,promoter analyses,protein localization,protein/protein interaction,DNA fragment modular assembly and functional verification of genomic fragments.Here we review research progress on the development of these destination vectors.
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    • References (42)
  • [1]
    耿立召,刘传亮,李付广.农杆菌介导法与基因枪轰击法结合在植物遗传转化上的应用[J].西北植物学报, 2005, 25(1): 205-210.
    [2]
    Hartley J L,Temple G F,Brasch M A.DNA cloning using in vitro site-specific recombination[J].Genome Res, 2000, 10: 1788-1795.
    [3]
    Karimi M,Depicker A,Hilson P.Recombinational cloning with plant gateway vectors[J].Plant Phy-siol, 2007, 145(4): 1144-1154.
    [4]
    Katzen F.Gateway recombinational cloning: a biological operating system[J].Expert Opin Drug Discov, 2007, 2(4): 571-589.
    [5]
    Cheo D L,Titus S A,Byrd D R N,Hartley J L,Temple G F,Brasch M A.Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones[J].Genome Res, 2004, 14: 2111-2120.
    [6]
    Sasaki Y,Sone T,Yoshida S,Yahata K,Hotta J,Chesnut J D,Honda T,Imamoto F.Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system[J].J Biotechnol, 2004, 107(3): 233-243.
    [7]
    Earley K W,Haag J R,Pontes O,Opper K,Juehne T,Song K,Pikaard C S.Gateway-compatible vectors for plant functional genomics and proteomics[J].Plant J, 2006, 45(4): 616-629.
    [8]
    Chen H,He H,Yu D.Overexpression of a novel soybean gene modulating Na+ and K+ transport enhances salt tolerance in transgenic tobacco plants[J].Physiol Plantarum, 2011, 141(1): 11-18.
    [9]
    Islam M R,Malo R,Tammi R S,Jahan S,Parvin L,Seraj Z I.Modification of recombination-based GatewayTM binary destination vector with novel promoter for Agrobacterium-mediated transformation of rice[J].Plant Tissue Cult & Biotech, 2008, 17(1): 47-58.
    [10]
    王峰,黄璐圆.双元Ti载体的发展[J].西北植物学报, 2006, 26(11): 2397-2401.
    [11]
    Curtis M D,Grossniklaus U.A gateway cloning vector set for high-throughput functional analysis of genes in planta[J].Plant Physiol, 2003, 133(2): 462-469.
    [12]
    Zuo J,Niu Q W,Chua N H.An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants[J].Plant J, 2000, 24(2): 265-273.
    [13]
    Joubès J,De Schutter K,Verkest A,Inzé D,De Veylder L.Conditional, recombinase-mediated expression of genes in plant cell cultures[J].Plant J, 2004, 37(6): 889-896.
    [14]
    Brand L,Hörler M,Nüesch E,Vassalli S,Barrell P,Yang W,Jefferson R A,Grossniklaus U,Curtis M D.A versatile and reliable two-component system for tissue-specific gene induction in Arabidopsis[J].Plant Physiol, 2006, 141(4): 1194-1204.
    [15]
    Helliwell C,Waterhouse P.Constructs and methods for high-throughput gene silencing in plants[J].Methods, 2003, 30(4): 289-295.
    [16]
    Karimi M,Inzé D,Depicker A.GatewayTM vectors for Agrobacterium-mediated plant transformation[J].Trends Plant Sci, 2002, 7(5): 193-195.
    [17]
    Lo C,Wang N,Lam E.Inducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco[J].FEBS Lett, 2005, 579(6): 1498-1502.
    [18]
    Bernacki S,Karimi M,Hilson P,Robertson N.Virus-induced gene silencing as a reverse genetics tool to study gene function[M].Plant Developmental Biology, 2010, 655: 27-45.
    [19]
    Liu Y,Schiff M,Dinesh-Kumar S P.Virus-induced gene silencing in tomato[J].Plant J, 2002, 31(6): 777-786.
    [20]
    Lacorte C,Ribeiro S G,Lohuis D,Goldbach R,Prins M.Potato virus X and tobacco mosaic virus-based vectors compatible with the GatewayTM cloning system[J].J Virol Methods, 2010, 164(1-2): 7-13.
    [21]
    Nakagawa T,Nakamura S,Tanaka K,Kawamukai M,Suzuki T,Nakamura K,Kimura T,Ishiguro S.Development of R4 Gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research[J].Biosci Biotech Bioch, 2008, 72(2): 624-629.
    [22]
    Xiao Y L,Redman J C,Monaghan E L,Zhuang J,Underwood B A,Moskal W A,Wang W,Wu H C,Town C D.High throughput generation of promoter reporter (GFP) transgenic lines of low expressing genes in Arabidopsis and analysis of their expression patterns[J].Plant Methods, 2010, 6: 18.
    [23]
    Nakagawa T,Kurose T,Hino T,Tanaka K,Kawamukai M,Niwa Y,Toyooka K,Matsuoka K,Jinbo T,Kimura T.Development of series of gateway binary vectors,pGWBs,for realizing efficient construction of fusion genes for plant transformation[J].J Biosci Bioeng, 2007, 104(1): 34-41.
    [24]
    Nakagawa T,Suzuki T,Murata S,Nakamura S,Hino T,Maeo K,Tabata R,Kawai T,Tanaka K,Niwa Y,Watanabe Y,Nakamura K,Kimura T,Ishiguro S.Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants[J].Biosci Biotech Bioch, 2007, 71(8): 2095-2100.
    [25]
    Nakamura S,Mano S,Tanaka Y,Ohnishi M,Nakamori C,Araki M,Niwa T,Nishimura M,Kaminaka H,Nakagawa T,Sato Y,Ishiguro S.Gateway binary vectors with the bialaphos resistance gene,bar,as a selection marker for plant transformation[J].Biosci Biotech Bioch, 2010, 74(6): 1315-1319.
    [26]
    Martin K,Kopperud K,Chakrabarty R,Banerjee R,Brooks R,Goodin M M.Transient expression in Nicotiana benthamiana fluorescent marker lines provides enhanced definition of protein localization,movement and interactions in planta[J].Plant J, 2009, 59(1): 150-162.
    [27]
    Ma L,Song Z B,Hu Q Q,Zhao Y,Nian H J,Yu Y X,Li K Z,Chen L M.Construction and application of a Gateway entry vector with rubisco small subunit promoter and its transit peptide sequence[J].Prog Biochem Biophys, 2011, 38(3): 269-279.
    [28]
    Song Z B,Orita I,Yin F,Yurimoto H,Kato N,Sakai Y,Izui K,Li K Z,Chen L M.Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde[J].Biotechnol Lett, 2010, 32(10): 1541-1548.
    [29]
    肖素勤,孙振,王莎莎,梁锋,李莉,年洪娟,李昆志,陈丽梅.通路克隆入门载体pEN-L4*-PrbcS-*T-gfp-L3*的构建及其应用[J].生命科学研究, 2011, 15(6): 476-484.
    [30]
    Xiao S Q,Sun Z,Wang S S,Zhang J,Li K Z,Chen L M.Overexpressions of dihydroxyacetone synthase and dihydroxyacetone kinase in chloroplasts install a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco using modified Gateway entry vectors[J].Acta Physiol Plant, 2012, 34(5): 1975-1985.
    [31]
    Rohila J S,Chen M,Cerny R,Fromm M E.Improved tandem affinity purification tag and methods for isolation of protein heterocomplexes from plants[J].Plant J, 2004, 38(1): 172-181.
    [32]
    Rubio V,Shen Y,Saijo Y,Liu Y,Gusmaroli G,Dinesh-Kumar S P,Deng X W.An alternative tandem affinity purification strategy applied to Arabidopsis protein complex isolation[J].Plant J, 2005, 41(5): 767-778.
    [33]
    Dubin M J,Bowler C,Benvenuto G.A modified Gateway cloning strategy for overexpressing tagged proteins in plants[J].Plant methods, 2008, 4: 3.
    [34]
    Van Leene J,Stals H,Eeckhout D,Persiau G,Van De Slijke E,Van Isterdael G,De Clercq A,Bonnet E,Laukens K,Remmerie N,Henderickx K, De Vijlder T,Abdelkrim A,De Jaeger G.A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana[J].Mol Cell Proteomics, 2007, 6: 1226-1238.
    [35]
    Zhong S,Lin Z F,Fray R G,Grierson D.Improved plant transformation vectors for fluorescent protein tagging[J].Transgenic Res, 2008, 17(5): 985-989.
    [36]
    Walter M,Chaban C,Schütze K,Batistic O,Weckermann K,Näke C,Blazevic D,Grefen C,Schumacher K,Oecking C,Harter K,Kudla J.Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation[J].Plant J, 2004, 40(3): 428-438.
    [37]
    Gehl C,Waadt R,Kudla J,Mendel R R,Hänsch R.New Gateway vectors for high throughput analyses of protein-protein interactions by bimolecular fluorescence complementation[J].Mol Plant, 2009, 2(5): 1051-1058.
    [38]
    Gehl C,Kaufholdt D,Hamisch D,Bikker R,Kudla J,Mendel R R,Hänsch R.Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors[J].Plant J, 2011, 67(3): 542-553.
    [39]
    Karimi M,De Meyer B,Hilson P.Modular cloning in plant cells[J].Trends Plant Sci, 2005, 10(3): 103-105.
    [40]
    Karimi M,Bleys A,Vanderhaeghen R,Hilson P.Building blocks for plant gene assembly[J].Plant Physiol, 2007, 145(4): 1183-1191.
    [41]
    Chen Q J,Zhou H M,Chen J,Wang X C.A Gateway-based platform for multigene plant transformation[J].Plant Mol Biol, 2006, 62(6): 927-936.
    [42]
    Ogawa Y,Dansako T,Yano K,Sakurai N,Suzuki H,Aoki K,Noji M,Saito K,Shibata D.Efficient and high-throughput vector construction and Agrobacterium-mediated transformation of Arabidopsis thaliana suspension-cultured cells for functional genomics[J].Plant Cell Physiol, 2008, 49(2): 242-250.
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