Advance Search
QIN Min, WU Ping-Ping, YAO Ming-Jing. Plant Regeneration from Different Explants of Perilla frutescens L.Britton[J]. Plant Science Journal, 2013, 31(2): 151-157. DOI: 10.3724/SP.J.1142.2013.20151
Citation: QIN Min, WU Ping-Ping, YAO Ming-Jing. Plant Regeneration from Different Explants of Perilla frutescens L.Britton[J]. Plant Science Journal, 2013, 31(2): 151-157. DOI: 10.3724/SP.J.1142.2013.20151
shu

Plant Regeneration from Different Explants of Perilla frutescens L.Britton

  • Institute of Medicinal Plant, College of Plant Science & Technology of Huazhong Agricultural University, Wuhan 430070, China

More Information
  • Received Date: May 15, 2012
  • Revised Date: September 17, 2012
  • Published Date: April 29, 2013
    Graphical Abstract
    • Abstract
  • Tissue culture and regeneration of the medicinal plant, Perilla frutescens, were investigated from different explants with different concentrations and combinations of hormones, and an efficient regeneration system was established. Explants showed high to low regeneration in the order terminal bud, lamina, cotyledon and hypocotyl. The regeneration rate of terminal bud was above 85% with or without hormone, and the addition of appropriate hormone promoted the proliferation rate. The regeneration rate of lamina was 70% on average with 0.05-0.1 mg/L TDZ. Optimal hormone combinations of cotyledon and hypocotyl were MS+KT 1.0 mg/L + IAA 1.0 mg/L and MS + 6-BA 1.0 mg/L + IAA 1.0 mg/L, with regeneration rates of 22.22% and 14.61%, respectively. The best rooting medium was 1/2 MS medium without hormone, leading to rooting rate of 86.67% and root length of 7.36 cm, on average. Explants from lamina could form leaf clusters, which induced adventitious buds repeatedly and proliferated quickly.
    • FullText(HTML)
    • References (14)
  • [1]
    方嘉禾, 常汝镇. 中国作物及其野生近缘植物: 经济作物卷[M]. 北京: [JP2]中国农业出版社, 2007: 222-223.
    [2]
    蒲海燕, 李影球, 李梅. 紫苏的功能性成分及其产品开发[J]. 中国食品添加剂, 2009(2): 133-137.
    [3]
    黄丹, 刘达玉. 紫苏提取物抑菌特性研究[J]. 食品工业, 2007(3): 11-13.
    [4]
    Kim J A, Choi H J, Park S K, Kim D U. Callus induction and plant regeneration from leaf segments and cotyledonary explants of Perilla frutescens[J]. Korean J Plant Tissue Cult, 1993, 20(1): 47-50.
    [5]
    Kim K H, Lee Y H, Kim D, Park Y H, Lee J Y, Hwang Y S, Kim Y H. Agrobacterium-mediated genetic transformation of Perilla frutescens[J]. Plant Cell Rep, 2004, 23(6): 386-390.
    [6]
    Hou S W, Jia J F. In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants[J]. Biol Plant, 2005, 49(1): 129-132.
    [7]
    Zhang T, Wang X Y, Cao Z Y. Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects[J]. Biol Plant, 2005, 49(3): 423-426.
    [8]
    牛炳韬. 紫苏离体再生体系的建立及体细胞无性系变异分析 [D]. 兰州:兰州大学, 2005.
    [9]
    黄海泉,张蕾,黄美娟. 紫苏组织培养及植株再生[J]. 亚热带植物科学, 2007, 36(4): 56.
    [10]
    于淑玲,石晓云. 紫苏组织培养体系的研究[J]. 邢台学院学报, 2008, 23(2): 118-125.
    [11]
    周川云, 戴向辰,胡彦. 紫苏不同外植体组培灭菌条件研究[J]. 广西农业科学, 2009, 40(4): 340-343.
    [12]
    刘培培. 紫苏离体再生体系的建立及其遗传转化初步研究[D].太原: 中北大学, 2011.
    [13]
    杨念, 曾宋君, 吴坤林, 陈之林, 段俊. 广藿香愈伤组织的诱导和增殖[J]. 福建林业科技, 2006, 33(3): 68-72.
    [14]
    Hossain H M M T, Kim Y H, Lee Y S. The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton[J]. Plant Biotechnol Rep, 2010, 4(3):229-235.
    • Related Articles

  • Related Articles

    [1]Yu Yue, Ran Guiping, Pi Limin. Nitrogen sensing and root development in plants[J]. Plant Science Journal, 2024, 42(6): 825-832. DOI: 10.11913/PSJ.2095-0837.24026
    [2]Zheng Chuan, Yang Ying-Zeng, Luo Xiao-Feng, Dai Yu-Jia, Liu Wei-Guo, Yang Wen-Yu, Shu Kai. Current understanding of the roles of phytohormone abscisic acid in the regulation of plant root growth[J]. Plant Science Journal, 2019, 37(5): 690-698. DOI: 10.11913/PSJ.2095-0837.2019.50690
    [3]Tang Yi-Xuan, Pi Li-Min, Zhu Yu-Xian. Epigenetic regulation of root stem cells in plants[J]. Plant Science Journal, 2019, 37(5): 682-689. DOI: 10.11913/PSJ.2095-0837.2019.50682
    [4]Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093
    [5]LI Yan, GONGYE Xiang-Xu, ZHU Hui-Jun, ZHAO Lei, YANG Yu-Qi, ZHANG Xing. Preliminary Study on Two-phase Culture of Tripterygium wilfordii Adventitious Root[J]. Plant Science Journal, 2016, 34(3): 469-474. DOI: 10.11913/PSJ.2095-0837.2016.30469
    [6]LI Dong-Xia, SHI Tao-Xiong, YUAN Pan, FENG Yan-Ni, SHI Lei. Differences in Root Microscopic Structure of Root Mutants lrn1, prl1 and Wild Type in Oilseed Rape (Brassica napus L.)[J]. Plant Science Journal, 2014, 32(4): 406-412. DOI: 10.3724/SP.J.1142.2014.40406
    [7]QIAO Qi, WEN Xiang-Ying, CHEN Hong-Feng, XING Fu-Wu. Ecological Anatomy of the Root in Bretschneidera sinensis,a Threatened Endemic Plant in China[J]. Plant Science Journal, 2010, 28(5): 544-549.
    [8]CUI Guang-Rong, YE Xuan-Yi, LIU Yue-Cheng, XIAO Ming, LIAO Ling-Ling, LIU Yue. The Types and Concentrations of Hormone Influence the Rate of Shoot Propagation and Rooting of Petunia hybrida[J]. Plant Science Journal, 2005, 23(4): 389-392.
    [9]SONG Li-Hong, CAO Bang-Hua. Studies on Activities of Indoleacetic Acid Oxidase, Polyphenol Oxidase and Peroxidase in Cuttings of Broussonetia papyrifera during Rooting Process[J]. Plant Science Journal, 2005, 23(4): 347-350.
    [10]Zhou Shigong, Jiao Genlin, Wu Zhaoming. ULTRASTRUCTURAL TYPE OF NUCLEOLUS IN ROOT MERISTEMATIC CELLS OF CUCUMIS SATIVUS L. AND ITS CHANGES UNDER BORON DEFICIENCY[J]. Plant Science Journal, 1989, 7(1): 55-58.

Catalog

    Get Citation
    PDF
    XML
    Article views (1668) PDF downloads (6254) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return