Advance Search
WANG Yao, HUANG Hui-Zhen, ZHANG Yong, YANG Bao-Yu, CHEN Shi-Yun. Isolation and Sequence Analysis of MARs from Populus nigra[J]. Plant Science Journal, 2007, 25(1): 24-28.
Citation: WANG Yao, HUANG Hui-Zhen, ZHANG Yong, YANG Bao-Yu, CHEN Shi-Yun. Isolation and Sequence Analysis of MARs from Populus nigra[J]. Plant Science Journal, 2007, 25(1): 24-28.

Isolation and Sequence Analysis of MARs from Populus nigra

More Information
  • Received Date: July 05, 2006
  • Revised Date: September 25, 2006
  • Published Date: February 12, 2007
  • In order to obtain matrix attachment regions(MARs) from woody plants,calli of Populus nigra were induced from young leaves,and suspension cultured cells were prepared from the calli and used as starting materials for MARs isolation.The nuclei were released after digestion of the cells with pectinase and cellulase,and nuclear matrixes were obtained after removal of the non-MARs segments.Separation of residual DNA and proteins were further performed by employing a high saline buffer,and the residual DNA was recovered and cloned.Two DNA fragments named A7 and A23 with typical MARs characteristics were obtained.Sequence analysis showed that both of the two isolated DNA fragments have the cha-racteristic motifs of MARs.Comparison with other known MARs sequences suggested that these two fragments were two new MARs isolated from Populus,and their functions were also predicted.To our know-ledge,this is the first report of MARs isolated from a woody plant.
  • Related Articles

    [1]Li Wei, Qiu Liwen, Wang Yimin, Peng Gangzhi, Gao Han, Li Zhengyan, Liu Lei. Flowering characteristics and breeding system of a remnant Myricaria laxiflora (Franch.) P. Y. Zhang et Y. J. Zhang population[J]. Plant Science Journal, 2024, 42(3): 296-303. DOI: 10.11913/PSJ.2095-0837.23211
    [2]Li Ji-Jun, Chen Ya-Hui, Zhou Zhi-Hua, Wang Yi-Jin, Yao Xuan, Guo Liang. Research progress on mechanisms of plant adaptation to flooding stress[J]. Plant Science Journal, 2023, 41(6): 835-846. DOI: 10.11913/PSJ.2095-0837.23234
    [3]Cao Meng-Hui, Zhao Qiu-Yan, Wei Chun-Mei, Huang Hai-Quan, Huang Mei-Juan. Study on the flowering traits and breeding systems of three Impatiens species[J]. Plant Science Journal, 2022, 40(3): 291-301. DOI: 10.11913/PSJ.2095-0837.2022.30291
    [4]Jiang Quan, Qiu Dong-Ping, Wang Zhi, Li Zuo-Zhou, Yao Xiao-Hong. Research progress on local adaptation in plants[J]. Plant Science Journal, 2021, 39(5): 559-570. DOI: 10.11913/PSJ.2095-0837.2021.50559
    [5]Wang Jie, Wei Ai-Li, Shi Ying, Li Yan-Hui, Han Yu-Xin, Wang Zhong-Jie. Adaptive evolutionary analysis of hetR gene in Nostoc[J]. Plant Science Journal, 2020, 38(1): 23-31. DOI: 10.11913/PSJ.2095-0837.2020.10023
    [6]Xu Kai, Cui Chang-Jie, Zou Pu, Ye Yu-Shi, Liao Jing-Ping, Kuang Yan-Feng, Liu Huan-Fang. Breeding system of Orchidantha chinensis var.longisepala[J]. Plant Science Journal, 2017, 35(4): 477-487. DOI: 10.11913/PSJ.2095-0837.2017.40477
    [7]Wang Hui, Li Xiao-Xia. Differentiation in breeding system and pollination of three sympatric Corydalis species[J]. Plant Science Journal, 2017, 35(2): 186-193. DOI: 10.11913/PSJ.2095-0837.2017.20186
    [8]Deng Hui, Xiang Gan-Ju, Guo You-Hao, Yang Chun-Feng. Study on the breeding system and floral color change of four Lonicera species in the Qinling mountains[J]. Plant Science Journal, 2017, 35(1): 1-12. DOI: 10.11913/PSJ.2095-0837.2017.10001
    [9]ZHAGN Yang, YE Qi-Gang. Breeding System of the Endangered Species Psilopeganum sinense and Its Pollination Process in a Botanical Garden[J]. Plant Science Journal, 2011, 1(5): 599-606.
    [10]Xu Naiyu. RESISTANT PLANTS BREEDING BY MEANS OF CELL/TISSUE CULTURE[J]. Plant Science Journal, 1987, 5(3): 299-302.

Catalog

    Article views (3290) PDF downloads (2129) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return