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YUAN Jing, SHU Qing-Yao, LIU Zhong-Lai. Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper[J]. Plant Science Journal, 2004, 22(3): 201-204.
Citation: YUAN Jing, SHU Qing-Yao, LIU Zhong-Lai. Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper[J]. Plant Science Journal, 2004, 22(3): 201-204.
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Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper

  • 1.

    Molecular Biology Laboratory, Central China Normal University, Wuhan 430079, China

  • 2.

    Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China

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  • Received Date: September 05, 2003
  • Revised Date: December 04, 2003
  • Published Date: June 25, 2004
    Graphical Abstract
    • Abstract
  • The insecticidal Bacillus thuringiensis(Bt)cryIAc gene was used to transform the cotyledon explants of pepper via Agrobacterium transformation.Through stringent selection of kanamycin,analysis of the expression of GUS in the plants by histochemical method,molecular biological assays with PCR and Southern dot hybridization,it is confirmed that the cryIAc gene was integrated into the genome of pepper.
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