Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant,Veronica ciliate Fisch.

To induce callus and establish rapid propagation systems for Veronica ciliate Fisch.,terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA.Results showed that the optimum medium for callus inducement was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,with the callus induction rate of 95%.The best medium for multiplication was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,with the multiplication coefficient reaching 5.4.The optimum root medium for multiple shoot clumps was 1/2 MS+IBA 0.05 mg/L,with the rooting rate reaching 95% and the root being healthiest.Under suitable hardening and transplanting conditions,the highest survival rate for the regenerated plantlets reached 40%.