2006 Vol. 24 No. 3
2006, 24(3): 191-195.
Abstract：
To study the structure-function relationships and the effect of the tryptophan residues to the CpeS of phycocyanin,two mutants,CpeS(W14I) and CpeS(W75S),were constructed.The lyase activity of these mutants were tested by reconstitution in vivo,to study the effects of tryptophans to CpeS.The results shows that:CpeS(W14I) has hardly lyase activity.Comparing with the wild CpeS,the lyase activity of CpeS(W14I) and CpeS(W75S) is respectively 8% and 76%.From this,it’s deduced that:the 14th tryptophan residue in CpeS may be essential amino acid to CpeS,and it may locate in the active site of CpeS.
To study the structure-function relationships and the effect of the tryptophan residues to the CpeS of phycocyanin,two mutants,CpeS(W14I) and CpeS(W75S),were constructed.The lyase activity of these mutants were tested by reconstitution in vivo,to study the effects of tryptophans to CpeS.The results shows that:CpeS(W14I) has hardly lyase activity.Comparing with the wild CpeS,the lyase activity of CpeS(W14I) and CpeS(W75S) is respectively 8% and 76%.From this,it’s deduced that:the 14th tryptophan residue in CpeS may be essential amino acid to CpeS,and it may locate in the active site of CpeS.
2006, 24(3): 196-200.
Abstract：
In this paper,the meiotic process of pollen mother cells and the somatic chromosome number 2n=32 of Pollia japonica Thunb.(Commelinaceae) are reported for the first time.The chromosomes in metaphase Ⅰ possess the configuration of 16Ⅱand the secondary association has also been observed.During anaphase Ⅰ and anaphaseⅡ,lagging chromosomes,chromosomal fragments and asynchronism have been observed and the abnormality rates of both phases are less than 10%.The rate of fertile pollen grains is more than 90%,which is consistent with the cell abnormality rate at anaphase Ⅱ.These results indicate that meiotic process of this species is normal,and the somatic chromosome number 2n=32 is credible.
In this paper,the meiotic process of pollen mother cells and the somatic chromosome number 2n=32 of Pollia japonica Thunb.(Commelinaceae) are reported for the first time.The chromosomes in metaphase Ⅰ possess the configuration of 16Ⅱand the secondary association has also been observed.During anaphase Ⅰ and anaphaseⅡ,lagging chromosomes,chromosomal fragments and asynchronism have been observed and the abnormality rates of both phases are less than 10%.The rate of fertile pollen grains is more than 90%,which is consistent with the cell abnormality rate at anaphase Ⅱ.These results indicate that meiotic process of this species is normal,and the somatic chromosome number 2n=32 is credible.
2006, 24(3): 201-206.
Abstract：
Genetic diversity and differentiation of populations of Thuidium cymbifolium between 3 planted spruce stands(PSS) and 3 naturally regenerated stands(NRS) were compared using random amplified polymorphic DNA(RAPD) marker.For PSS and NRS,the average percentage of polymorphic loci(PPL=12.7%,10.0%),Nei’s genetic diversity(HE = 0.042,0.027) and Shannon’s information index(S=0.064,0.043) were revealed by POPGENE,respectively.The results of population differentiation among the populations from PSS(Gst=0.592,Fst=0.639) and NRS(Gst=0.702,Fst= 0.695),together with the results of UPGMA cluster analysis and of principal components analysis showed that gene flow between populations was limited.AMOVA revealed a significantly higher level of population genetic diversity existed in PSS than that in NRS,which indicated that artificial reforestation after forest clearcut was effective in promoting the recovery of genetic diversity of the recolonized moss species.
Genetic diversity and differentiation of populations of Thuidium cymbifolium between 3 planted spruce stands(PSS) and 3 naturally regenerated stands(NRS) were compared using random amplified polymorphic DNA(RAPD) marker.For PSS and NRS,the average percentage of polymorphic loci(PPL=12.7%,10.0%),Nei’s genetic diversity(HE = 0.042,0.027) and Shannon’s information index(S=0.064,0.043) were revealed by POPGENE,respectively.The results of population differentiation among the populations from PSS(Gst=0.592,Fst=0.639) and NRS(Gst=0.702,Fst= 0.695),together with the results of UPGMA cluster analysis and of principal components analysis showed that gene flow between populations was limited.AMOVA revealed a significantly higher level of population genetic diversity existed in PSS than that in NRS,which indicated that artificial reforestation after forest clearcut was effective in promoting the recovery of genetic diversity of the recolonized moss species.
2006, 24(3): 205-252.
Abstract：
To establish parameters for identification of the medicinal material of two types of Selaginella heterostachys,several methods,including the morphological,microscopic and near infrared spectroscopic ones,were used in this study.The two types of S.heterostachys differ obviously in the morphological characters,but there are few differences in the spore appearance,histological characters of the stems,and the near infrared spectra.The results provided basis for classification of the species and identification of the medicinal material.
To establish parameters for identification of the medicinal material of two types of Selaginella heterostachys,several methods,including the morphological,microscopic and near infrared spectroscopic ones,were used in this study.The two types of S.heterostachys differ obviously in the morphological characters,but there are few differences in the spore appearance,histological characters of the stems,and the near infrared spectra.The results provided basis for classification of the species and identification of the medicinal material.
2006, 24(3): 207-211.
Abstract：
’Wild cucumber’(Cucumis hystrix,2n=24) is a short-day plant.The hybridization between wild and cultivated cucumber(C.sativus,2n=14) is hard to be made because of their differential flowering time.In the present study,the flowering time was synchronized by short day treatment of the C.hystrix for over 40 days.The crosses were made between C.hystrix and three different genotypic cultivated cucumbers(C.sativus cv.Changchun mici,NC4406,and Erzaozi) and the hybrid embryos were obtained.Using our modified embryo rescuing methods,the embryo regeneration frequency reached 80%.The three interspecific hybrids have been identified through morphological characterization,chromosome count and aspartate aminotransferase(AAT) analyses.Varied levels of fertility were observed among these hybrids.The stain ability of pollen grains in C.hystrix×NC4406 was 23.3%,highest in the varions combinations,which implied that the hybrid Cucumis hystrix×NC4406 might be a better germplasm for transferring exotic genes at the diploid level.
’Wild cucumber’(Cucumis hystrix,2n=24) is a short-day plant.The hybridization between wild and cultivated cucumber(C.sativus,2n=14) is hard to be made because of their differential flowering time.In the present study,the flowering time was synchronized by short day treatment of the C.hystrix for over 40 days.The crosses were made between C.hystrix and three different genotypic cultivated cucumbers(C.sativus cv.Changchun mici,NC4406,and Erzaozi) and the hybrid embryos were obtained.Using our modified embryo rescuing methods,the embryo regeneration frequency reached 80%.The three interspecific hybrids have been identified through morphological characterization,chromosome count and aspartate aminotransferase(AAT) analyses.Varied levels of fertility were observed among these hybrids.The stain ability of pollen grains in C.hystrix×NC4406 was 23.3%,highest in the varions combinations,which implied that the hybrid Cucumis hystrix×NC4406 might be a better germplasm for transferring exotic genes at the diploid level.
2006, 24(3): 212-212.
Abstract：
Based on examinations of syntype specimens housed at WUK,the lectotype for the name Rubia ovatifolia Z.Y.Zhang is designated here.
Based on examinations of syntype specimens housed at WUK,the lectotype for the name Rubia ovatifolia Z.Y.Zhang is designated here.
2006, 24(3): 213-214.
Abstract：
In the past several years,the author studied the bryoflora of Xinjiang,Northwest China and increased a series of new records there.Pylaisia steerei(Ando et Higuchi) Ignatov,as a new addition to the Flora Bryophytorum Sinicorum was described and illustrated.
In the past several years,the author studied the bryoflora of Xinjiang,Northwest China and increased a series of new records there.Pylaisia steerei(Ando et Higuchi) Ignatov,as a new addition to the Flora Bryophytorum Sinicorum was described and illustrated.
2006, 24(3): 215-219.
Abstract：
Scientific survey and identification of plant species indicate that the seed plants are made up of 285 species(which belong to 103 genera and 33 families) in the alpine frigid zone in Shergyla Mountain of Tibet.Among them there are 236 species in 26 families,77 genera of dicotyledons and 4 species(in 1 families,1 genus) of gymnospermae,and 125 species endemic to China.Based on the analysis of the floristic elements from the levels of species,genus and family rank,the results show that:the flora is simple in geographical elements,evident in endemism,and lacking of the ancient and primitive forms in composition.On the analysis of family,genus and species area-types,the component of this flora is basically composed from temperate ones.It shows that the flora is not only of typical temperate character,but also of youthful character,so the flora is a typical alpine frigid one.Owing to the elements in the flora are mainly of Sino-Himalayan ones,so the flora is obviously with plateau and alpine floristic features.
Scientific survey and identification of plant species indicate that the seed plants are made up of 285 species(which belong to 103 genera and 33 families) in the alpine frigid zone in Shergyla Mountain of Tibet.Among them there are 236 species in 26 families,77 genera of dicotyledons and 4 species(in 1 families,1 genus) of gymnospermae,and 125 species endemic to China.Based on the analysis of the floristic elements from the levels of species,genus and family rank,the results show that:the flora is simple in geographical elements,evident in endemism,and lacking of the ancient and primitive forms in composition.On the analysis of family,genus and species area-types,the component of this flora is basically composed from temperate ones.It shows that the flora is not only of typical temperate character,but also of youthful character,so the flora is a typical alpine frigid one.Owing to the elements in the flora are mainly of Sino-Himalayan ones,so the flora is obviously with plateau and alpine floristic features.
2006, 24(3): 220-224.
Abstract：
Danjiangkou Reservoir is the water supplying area of the Middle-Route of South-to-North Water Transfers Project.To serve that purpose,the existing Danjiangkou Dam will be elevated to the level of 176.6 m(above sea level) from 162 m,and the normal pool level will be 170 m instead of the present 157 m.Thus,analysis for the composition and distribution of existing vegetation in Danjiangkou Reservoir Region will provide data for wetland management and water environment maintenance of the future.The present paper detected the distribution patterns and species composition of the wetland vegetations using TWINSPAN and DCA in the Danjiangkou Reservoir Region.TWINSPAN classification divided the vegetation into 14 associations,and their community characteristics were described.DCA ordination of samples revealed that soil type and humidity were the main factors influencing the structure and distribution of wetland community.The current floodplain vegetation in Danjiangkou Reservoir was dominated by Cy-nodon dactylon,Rumex maritimus,Mazus gracili and Echinochloa crusgalli.
Danjiangkou Reservoir is the water supplying area of the Middle-Route of South-to-North Water Transfers Project.To serve that purpose,the existing Danjiangkou Dam will be elevated to the level of 176.6 m(above sea level) from 162 m,and the normal pool level will be 170 m instead of the present 157 m.Thus,analysis for the composition and distribution of existing vegetation in Danjiangkou Reservoir Region will provide data for wetland management and water environment maintenance of the future.The present paper detected the distribution patterns and species composition of the wetland vegetations using TWINSPAN and DCA in the Danjiangkou Reservoir Region.TWINSPAN classification divided the vegetation into 14 associations,and their community characteristics were described.DCA ordination of samples revealed that soil type and humidity were the main factors influencing the structure and distribution of wetland community.The current floodplain vegetation in Danjiangkou Reservoir was dominated by Cy-nodon dactylon,Rumex maritimus,Mazus gracili and Echinochloa crusgalli.
2006, 24(3): 225-230.
Abstract：
The effects of temperature,light and pH on photosynthesis of Microcystis aeruginosa and Chloromonas rosae were studied by measuring their net photosynthetic O2 evolutions.The net photosynthesis of both algae increase with the temperature.The net photosynthetic O2 evolution of M.aeruginosa increased linearly in the range of 10-35℃,indicating that its optimal temperature might be higher than 35℃,while the net photosynthetic O2 evolution of Ch.rosae decreased dramatically when the temperature was over 30℃.The photosynthesis-light response curves of these two algae are different.The light saturation point(LSP) of M.aeruginosa was around 500 μmol·m-2·s-1 and photoinhibition did not occur when the light intensity was as high as 900 μmol·m-2·s-1,much different from M.aeruginosa,photosynthesis of Ch.rosae was inhibited when the light intensity exceeded its LSP of 630 μmol·m-2·s-1.The photosynthesis of M.aeruginosa,with an optimal pH of 10.0,was very active and stable in the range of pH 6.5-11.5.The optimal pH of Ch.rosae was 7.0,and any slight departure from the optimal pH would significantly reduce its photosynthesis.When the value of pH exceeded 10.0,its net photosynthe-tic O2 evolution was negative.M.aeruginosa has three important characteristics that are different from Ch.rosae:(1) a wider tolerable temperature range,adaptation to high temperature;(2) lower LSP,higher photosynthesis activity,and more efficient photosynthesis under low light environment;(3) significant pH tolerance,and high photosynthesis activity in neutral and alkaline environment.All these biological characteristics give M.aeruginosa great competition advantages to survive,grow and dominate other phytoplankton in polluted aquatic environment.
The effects of temperature,light and pH on photosynthesis of Microcystis aeruginosa and Chloromonas rosae were studied by measuring their net photosynthetic O2 evolutions.The net photosynthesis of both algae increase with the temperature.The net photosynthetic O2 evolution of M.aeruginosa increased linearly in the range of 10-35℃,indicating that its optimal temperature might be higher than 35℃,while the net photosynthetic O2 evolution of Ch.rosae decreased dramatically when the temperature was over 30℃.The photosynthesis-light response curves of these two algae are different.The light saturation point(LSP) of M.aeruginosa was around 500 μmol·m-2·s-1 and photoinhibition did not occur when the light intensity was as high as 900 μmol·m-2·s-1,much different from M.aeruginosa,photosynthesis of Ch.rosae was inhibited when the light intensity exceeded its LSP of 630 μmol·m-2·s-1.The photosynthesis of M.aeruginosa,with an optimal pH of 10.0,was very active and stable in the range of pH 6.5-11.5.The optimal pH of Ch.rosae was 7.0,and any slight departure from the optimal pH would significantly reduce its photosynthesis.When the value of pH exceeded 10.0,its net photosynthe-tic O2 evolution was negative.M.aeruginosa has three important characteristics that are different from Ch.rosae:(1) a wider tolerable temperature range,adaptation to high temperature;(2) lower LSP,higher photosynthesis activity,and more efficient photosynthesis under low light environment;(3) significant pH tolerance,and high photosynthesis activity in neutral and alkaline environment.All these biological characteristics give M.aeruginosa great competition advantages to survive,grow and dominate other phytoplankton in polluted aquatic environment.
2006, 24(3): 231-234.
Abstract：
Three cultivars of sweet Osmanthus,including O.fragrans ’Ruanye Dangui’,’Liuye Yingui’ and ’Sijigui’ were conducted to study the changes of pigment,soluble sugar and protein of petals of different cultivars during the period of their florescence and senescence.The results showed as follows:(1)It is the same with these three different cultivars that during the whole florescence period from the initial stage to the middle stage,then to the late stage,the content of carotenoid and anthocyanidin experienced an obvious change of firstly increasing and then decreasing,which is consistent with the change of the color of the petal,showing that the changes of the color of the petal during the florescence were mainly caused by the change of the pigment.(2)The content of water,soluble sugar and soluble protein in the petals of these three cultivars decreased obviously during their florescence and senescence,and the decreasing of those in ’Ruanye Dangui’,which had a longer anthesis was slower than the other two cultivars,while the decreasing between ’Liuye Yingui’ and ’Sijigui’ was not significantly different.
Three cultivars of sweet Osmanthus,including O.fragrans ’Ruanye Dangui’,’Liuye Yingui’ and ’Sijigui’ were conducted to study the changes of pigment,soluble sugar and protein of petals of different cultivars during the period of their florescence and senescence.The results showed as follows:(1)It is the same with these three different cultivars that during the whole florescence period from the initial stage to the middle stage,then to the late stage,the content of carotenoid and anthocyanidin experienced an obvious change of firstly increasing and then decreasing,which is consistent with the change of the color of the petal,showing that the changes of the color of the petal during the florescence were mainly caused by the change of the pigment.(2)The content of water,soluble sugar and soluble protein in the petals of these three cultivars decreased obviously during their florescence and senescence,and the decreasing of those in ’Ruanye Dangui’,which had a longer anthesis was slower than the other two cultivars,while the decreasing between ’Liuye Yingui’ and ’Sijigui’ was not significantly different.
2006, 24(3): 235-239.
Abstract：
The effects of different storage temperatures on lignification and related enzyme activities of loquat(Eriobotrya japonica Lindl.cv.Jiefangzhong) pulp stored at 4℃ and 12℃ were studied. The results showed that phenylalanine ammonia lyase(PAL,EC 4.1.3.5.) activity, 4-coumarate coenzyme A ligase(4-CL,EC 6.2.1.12.) activity,cinnamyl alcohol dehydrogenase(CAD,EC 1.1.1.195.) activity and peroxidase(POD,EC 1.11.1.7.) activity of loquat pulp stored at 4℃ were higher than those stored at 12℃,while polyphenol oxidase(PPO,EC 1.10.3.2.) activity was lower.The firmness and lignin content of loquat pulp at 4℃ increased during storage,which results in comparatively serious lignification in loquat pulp.The firmness and lignin content of loquat pulp at 12℃ were lower than at 4℃,hence a lo-wer level of lignification in loquat pulp.The main factor for lignification in loquat pulp is that the low temperature caused higher phenylalanine ammonia lyase activity and peroxidase activity.
The effects of different storage temperatures on lignification and related enzyme activities of loquat(Eriobotrya japonica Lindl.cv.Jiefangzhong) pulp stored at 4℃ and 12℃ were studied. The results showed that phenylalanine ammonia lyase(PAL,EC 4.1.3.5.) activity, 4-coumarate coenzyme A ligase(4-CL,EC 6.2.1.12.) activity,cinnamyl alcohol dehydrogenase(CAD,EC 1.1.1.195.) activity and peroxidase(POD,EC 1.11.1.7.) activity of loquat pulp stored at 4℃ were higher than those stored at 12℃,while polyphenol oxidase(PPO,EC 1.10.3.2.) activity was lower.The firmness and lignin content of loquat pulp at 4℃ increased during storage,which results in comparatively serious lignification in loquat pulp.The firmness and lignin content of loquat pulp at 12℃ were lower than at 4℃,hence a lo-wer level of lignification in loquat pulp.The main factor for lignification in loquat pulp is that the low temperature caused higher phenylalanine ammonia lyase activity and peroxidase activity.
2006, 24(3): 240-244.
Abstract：
The tube broth method and the cup-plate method were adopted to study antimicrobial activity in vitro of the extracts from Fagopyrum dibotrys.The results suggested that F.dibotrys extracts have strong antibacterial action in vitro,and the extracts have high inhibition on Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Bacillus thuringiensis,Diplococcus catarrhalis,Sclerophthora macrospora,Candida albicans,Pestalotia funereal,Rhizoctonia solani,Sclerotinia sclerotiorum,Curvularia lunata(Walk) Boed,Gibberelle zeae(Schw) and Trichoderma viride.
The tube broth method and the cup-plate method were adopted to study antimicrobial activity in vitro of the extracts from Fagopyrum dibotrys.The results suggested that F.dibotrys extracts have strong antibacterial action in vitro,and the extracts have high inhibition on Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Bacillus thuringiensis,Diplococcus catarrhalis,Sclerophthora macrospora,Candida albicans,Pestalotia funereal,Rhizoctonia solani,Sclerotinia sclerotiorum,Curvularia lunata(Walk) Boed,Gibberelle zeae(Schw) and Trichoderma viride.
2006, 24(3): 245-249.
Abstract：
The differences of leaf epidermis of ’Nublue’ and ’Kenblue’,two varieties of Poa pratensis L.under two habitats which were the vegetation concrete,and the rural area soil were studied in this paper.Thus there were four samples totally,four parameters were concerned,which were the length of long cell,the length of short cell,the length/width ratio of gas vent and the numbers of gas vent per field of vision.It showed remarkable difference in the length of long cell,the length of short cell,the length/width ratio of gas vent between each group.As the vegetation concrete was a kind of alkaline environment,it was reaso-nable to conclude that these two varieties had relatively greater plasticity to the alkaline environment.In comparison of gas vent numbers,’Nublue’ showed remarkable difference,which demonstrated that it had a relatively large plasticity to the changes of moisture and gas,while ’Kenblue’ showed little difference which showed its greater adaptability to the changes of moisture and gas.
The differences of leaf epidermis of ’Nublue’ and ’Kenblue’,two varieties of Poa pratensis L.under two habitats which were the vegetation concrete,and the rural area soil were studied in this paper.Thus there were four samples totally,four parameters were concerned,which were the length of long cell,the length of short cell,the length/width ratio of gas vent and the numbers of gas vent per field of vision.It showed remarkable difference in the length of long cell,the length of short cell,the length/width ratio of gas vent between each group.As the vegetation concrete was a kind of alkaline environment,it was reaso-nable to conclude that these two varieties had relatively greater plasticity to the alkaline environment.In comparison of gas vent numbers,’Nublue’ showed remarkable difference,which demonstrated that it had a relatively large plasticity to the changes of moisture and gas,while ’Kenblue’ showed little difference which showed its greater adaptability to the changes of moisture and gas.
Abstract：
The Houhe National Nature Reserve(HNNR) is rich of biodiversity,and as a part of the key region of biodiversity from the eastern Sichuan to the western Hubei and northwestern Hunan,which is one of the three biodiversity centers in China.However,biodiversity of HNNR was damaged in the past years mainly by deforestation and hunting,since Wufeng is a poverty mountainous county and forest industries has been its main economic pillar for a long time.Fortunately,with the establishment and development of the reserve, the biodiversity richness has been effectively protected,especially after its upgrading to national level in 2000,the effort to conservation strategy in HNNR has been greatly enhanced.At the same time,biodiversity in HNNR is still facing threat from various factors.The shortage of research personnel and small size of its area have limited advanced study and conservation of the biodiversity in the reserve.For the future conservation and recovery of the biodiversity,introducing well-trained research personnel and improving the research facilities,enlarging the areas of the reserve and advancing the community co-management are the highest priorities for the moment.
The Houhe National Nature Reserve(HNNR) is rich of biodiversity,and as a part of the key region of biodiversity from the eastern Sichuan to the western Hubei and northwestern Hunan,which is one of the three biodiversity centers in China.However,biodiversity of HNNR was damaged in the past years mainly by deforestation and hunting,since Wufeng is a poverty mountainous county and forest industries has been its main economic pillar for a long time.Fortunately,with the establishment and development of the reserve, the biodiversity richness has been effectively protected,especially after its upgrading to national level in 2000,the effort to conservation strategy in HNNR has been greatly enhanced.At the same time,biodiversity in HNNR is still facing threat from various factors.The shortage of research personnel and small size of its area have limited advanced study and conservation of the biodiversity in the reserve.For the future conservation and recovery of the biodiversity,introducing well-trained research personnel and improving the research facilities,enlarging the areas of the reserve and advancing the community co-management are the highest priorities for the moment.
2006, 24(3): 261-266.
Abstract：
Proteomics which is the new discipline in the time of the post-genomics develops rapidly in the life science.Techniques of separation and detection in proteomics are the important foundation in promoting the development of proteomics.The present paper has documented the current situation and new development of the techniques of separation and detection in this area,including 2-dimensional gel electrophoresis,chromatography and mass spectrometry.It also summarized our lab’s work on proteomics assisted by bioinformatics.
Proteomics which is the new discipline in the time of the post-genomics develops rapidly in the life science.Techniques of separation and detection in proteomics are the important foundation in promoting the development of proteomics.The present paper has documented the current situation and new development of the techniques of separation and detection in this area,including 2-dimensional gel electrophoresis,chromatography and mass spectrometry.It also summarized our lab’s work on proteomics assisted by bioinformatics.
2006, 24(3): 267-270.
Abstract：
The vessel elements of secondary xylem in Ziziphus mauritiana was observed by bio-microscope image analysis system and micrography.The results show that there are various patterns of vessel elements and three types of perforation plates: both ends of the vessel element aresimple perforation plate;one end is simple perforation and the other is scalariform perforation;the simple perforation with cilia in Z.mauritiana.It is discussed that the ontogeny and phylogenesis of vessel elements in Z.mauritiana.The new evidence was provide on the phylogenesis of perforation.
The vessel elements of secondary xylem in Ziziphus mauritiana was observed by bio-microscope image analysis system and micrography.The results show that there are various patterns of vessel elements and three types of perforation plates: both ends of the vessel element aresimple perforation plate;one end is simple perforation and the other is scalariform perforation;the simple perforation with cilia in Z.mauritiana.It is discussed that the ontogeny and phylogenesis of vessel elements in Z.mauritiana.The new evidence was provide on the phylogenesis of perforation.
2006, 24(3): 271-276.
Abstract：
The different developmental phases of leaves in Polygonatum cyrtonema and P.anhuiense are studied in this paper.The results are as follows:(1) Stomatal apparatus indexes are both from low to high,when reach to max,then gradually reduce to stabilization.(2) The tendency of length and width ratio of leaf in both P.cyrtonema and P.anhuiense are going up,and which has relativity with leaf shape,but there is no any obvious relativity in length and width ratio of leaf and epidermis cell.(3) The styles of development of leaf epidermis are the same,which are the processes of growth in width and length.(4) In the process of leaf differentiation,the first emergency is epidermis,then mesophyll,at last is vein.(5) The leaf margin of P.cyrtonema have many big parenchyma cells in mesophyll at the early developmental stage,then disappeared or continue existed when leaf mature,but in P.anhuiense,there is no big parenchyma cell appear in all developmental processes.Those studies accumulate experimental data for studies on leaf development of whorled leaf and alternate leaf in Polygonatum Mill.
The different developmental phases of leaves in Polygonatum cyrtonema and P.anhuiense are studied in this paper.The results are as follows:(1) Stomatal apparatus indexes are both from low to high,when reach to max,then gradually reduce to stabilization.(2) The tendency of length and width ratio of leaf in both P.cyrtonema and P.anhuiense are going up,and which has relativity with leaf shape,but there is no any obvious relativity in length and width ratio of leaf and epidermis cell.(3) The styles of development of leaf epidermis are the same,which are the processes of growth in width and length.(4) In the process of leaf differentiation,the first emergency is epidermis,then mesophyll,at last is vein.(5) The leaf margin of P.cyrtonema have many big parenchyma cells in mesophyll at the early developmental stage,then disappeared or continue existed when leaf mature,but in P.anhuiense,there is no big parenchyma cell appear in all developmental processes.Those studies accumulate experimental data for studies on leaf development of whorled leaf and alternate leaf in Polygonatum Mill.
2006, 24(3): 277-280.
Abstract：
The research was about the effects of enhanced UV-B radiation on chlorophyll content of two wild sugarcane(Saccharum spontaneum L.) clones for two consecutive years in field condition.The results showed that clone 93-25 was more resistent than clone 92-11.Chlorophyll content of clone 92-11 decreased while that of clone 93-25 increased under 5 kJ/m2 UV-B radiation.Interspecific difference in chlorophyll content of wide sugarcanes existed.Clone 92-11 was more resistent in 2004 than 2003 while clone 93-25 was opposite change.The clones were more sensitive in their flowering stage than tillering stage,and the elongating stage was the most resistent stage.
The research was about the effects of enhanced UV-B radiation on chlorophyll content of two wild sugarcane(Saccharum spontaneum L.) clones for two consecutive years in field condition.The results showed that clone 93-25 was more resistent than clone 92-11.Chlorophyll content of clone 92-11 decreased while that of clone 93-25 increased under 5 kJ/m2 UV-B radiation.Interspecific difference in chlorophyll content of wide sugarcanes existed.Clone 92-11 was more resistent in 2004 than 2003 while clone 93-25 was opposite change.The clones were more sensitive in their flowering stage than tillering stage,and the elongating stage was the most resistent stage.
2006, 24(3): 281-285.
Abstract：
Thirty varieties of non-heading Chinese cabbage were treated with low light intensityand classified with the cluster analysis by three indices,change of dry weight,change of leaf thick and change of chl.a/b.The thirty varieties were divided into three basic types,tolerant,middle-tolerant and susceptible.Among these varieties,nine were susceptible,fifteen were middle-tolerant,and six were tolerant.The results showed that the correlation of three indices were significant.Comparatively,the varieties Shulü,Zhengda Kangreqing No.3,Reyou No.2,Kangre No.605,Aiwang and Huawang were more tolerant.
Thirty varieties of non-heading Chinese cabbage were treated with low light intensityand classified with the cluster analysis by three indices,change of dry weight,change of leaf thick and change of chl.a/b.The thirty varieties were divided into three basic types,tolerant,middle-tolerant and susceptible.Among these varieties,nine were susceptible,fifteen were middle-tolerant,and six were tolerant.The results showed that the correlation of three indices were significant.Comparatively,the varieties Shulü,Zhengda Kangreqing No.3,Reyou No.2,Kangre No.605,Aiwang and Huawang were more tolerant.
