Advance Search
WANG Ying-Li, HUANG Wen-Jun, WANG Ying. Cloning and Expression Analysis of the EsUF3GT Gene in Epimedium sagittatum (Sieb. and Zucc.) Maxim.[J]. Plant Science Journal, 2014, 32(6): 602-611. DOI: 10.11913/PSJ.2095-0837.2014.60602
Citation: WANG Ying-Li, HUANG Wen-Jun, WANG Ying. Cloning and Expression Analysis of the EsUF3GT Gene in Epimedium sagittatum (Sieb. and Zucc.) Maxim.[J]. Plant Science Journal, 2014, 32(6): 602-611. DOI: 10.11913/PSJ.2095-0837.2014.60602
shu

Cloning and Expression Analysis of the EsUF3GT Gene in Epimedium sagittatum (Sieb. and Zucc.) Maxim.

  • 1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;

  • 2. University of Chinese Academy of Sciences, Beijing 100049, China

More Information
  • Received Date: February 11, 2014
  • Available Online: October 31, 2022
  • Published Date: December 29, 2014
    Graphical Abstract
    • Abstract
  • Flavonoid 3-O-glycosyltransferase (UF3GT) can catalyze unstable anthocyanidin into anthocyanin. In this study, the Open Reading Frame (ORF) sequence of a novel UF3GT gene, designated as EsUF3GT, was isolated from Epimedium sagittatum (Sieb. and Zucc.) Maxim. using homologous gene cloning. The sequence was logged into the GenBank database with an accession No. of KJ648620. Its cDNA sequence contained a 1356 bp complete ORF, encoding 451 amino acids, and the deduced amino acid showed 40%-50% homology with other plant UF3GTs. Phylogenetic analysis revealed that EsUF3GT had a close relationship with glycosyltransferases (GTs) that catalyze the flavonoid 3-O glycosylation from various species. qRT-PCR analysis indicated that EsUF3GT showed the highest expression level in flowers, about 2.3 times that found in leaves and flower buds, and 19 times that found in the fruit and roots. Anthocyanin showed the highest accumulation level of 130.4 mg/100 g in flower buds, about 3.5, 5.2, 72 and 87 times that found in leaves, flowers, fruits and roots, respectively. We speculated that EsUF3GT was involved in anthocyanin biosynthesis in E. sagittatum. These results lay a foundation for further exploration of the function of EsUF3GT.
    • FullText(HTML)
    • References (43)
  • [1]
    Stearn WT, Shaw JMH. The Genus Epimedium and Other Herbaceous Berberidaceae[M]. Portland: Timber Press Inc., 2004: 202-203.
    [2]
    Ma HP, He XR, Yang Y, Li MX, Hao DG, Jia ZP. The genus Epimedium: An ethnopharmacological and phytochemical review[J]. J Ethnopharmacol, 2011, 134: 519-541.
    [3]
    国家药典委员会. 中华人民共和国药典(一部)[S]. 北京:中国医药科技出版社, 2010: 306-308.
    [4]
    黄秀兰, 周亚伟, 王伟. 淫羊藿黄酮类化合物药理研究进展[J]. 中成药, 2005, 27(6): 719-721.
    [5]
    任璘, 戴思兰, 王瑛. 淫羊藿属植物种质资源及其园林应用[J]. 武汉植物学研究, 2008, 26(6): 644-649.
    [6]
    Winkel-Shiley B. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology[J]. Plant Physiol, 2001, 126(2): 485-493.
    [7]
    于晓南, 张启翔. 观赏植物的花色素苷与花色[J]. 林业科学, 2002, 38(3): 148-152.
    [8]
    Forkmann G. Flavonoids as flower pigments: the formation of the natural spectrum and its extension by genetic engineering[J]. Plant Breeding, 1991, 106(1): 1-26.
    [9]
    Springob K, Nakajima JI, Yamazaki M, Saito K. Recent advances in the biosynthesis and accumulation of anthocyanins[J]. Nat Prod Rep, 2003, 20(3): 288-303.
    [10]
    Sakakibara KY, Nakayama T, Yamazaki M, Saito K. Modification and Stabilization of Anthocyanins[M]. New York: Springer, 2009: 169-190.
    [11]
    侯夫云, 王庆美, 李爱贤, 张海燕, 董顺旭, 解备涛. 植物花青素合成酶的研究进展[J]. 中国农学通报, 2009, 25(21): 188-190.
    [12]
    Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima JI, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K. Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor[J]. Plant J, 2005, 42(2): 218-235.
    [13]
    王军, 侯丙凯. 植物小分子化合物的糖基化与糖基转移酶[J]. 植物生理学通讯, 2008, 44(5): 997-1003.
    [14]
    Goto T, Kondo T, Tamura H, Imagawa H. Structure of gentiodelphin, an acylated anthocyanin isolated from Gentiana makinoi, that is stable in dilute aqueous solution[J]. Tetrahedron Lett, 1982, 23(36): 3695-3698.
    [15]
    Ford CM, Boss PK, Hoj PB. Cloning and characterization of Vitis vinifera UDP-Glucose: Flavonoid 3-O-Glucosyltransferase, a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo[J]. J Biol Chem, 1998, 273(15): 9224-9233.
    [16]
    Hall D, Yuan XX, Murata J, Luca VD. Molecular cloning and biochemical characterization of the UDP-glucose: Flavonoid 3-O-glucosyltransferase from Concord grape (Vitis labrusca) [J]. Phytochemistry, 2012, 74(4): 90-99.
    [17]
    Tanaka Y, Yoshikazu K, Fukuchi-Mizutani M, Fukui Y, Fujiwara H, Ashikari T, Kusumi T. Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora[J]. Plant Cell Physiol, 1996, 37(5): 711-716.
    [18]
    Yamazaki M, Yamagishi E, Gong ZZ, Mizutani MF, Fukui Y, Tanaka Y, Kusumi T, Yamaguchi M, Saito K. Two avonoid glucosyltransferases from Petunia hybrida: molecular cloning, biochemical properties and developmentally regulated expression[J]. Plant Mol Biol, 2002, 48(4): 401-411.
    [19]
    李作洲,徐艳琴,王瑛,黄宏文. 淫羊藿属药用植物的研究现状与展望[J]. 中草药, 2005, 36(2): 289-295.
    [20]
    Zeng SH, Xiao G, Guo J, Fei ZJ, Xu YQ, Roe BA, Wang Y. Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant, Epimedium sagittatum (Sieb. and Zucc.) Maxim.[J]. BMC genomics, 2010, 11(1): 94-95.
    [21]
    Offen W, Fleites CM, Yang M, Lim EK, Davis BG, Tarling CA, Ford CM, Bowles DJ, Davies GJ. Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modication[J]. EMBO J, 2006, 25(6): 1396-1405.
    [22]
    Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408.
    [23]
    Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. Analysis and biological activities of anthocyanins[J]. Phytochemistry, 2003, 64(5): 923-933.
    [24]
    Nakajima JI, Tanaka Y, Yamazaki M, Saito K. Reaction mechanism from leucoanthocyanidin to anthocyanidin 3-glucoside, a key reaction for coloring in anthocyanin biosynthesis[J]. J Biol Chem, 2001, 276(28): 25797-25803.
    [25]
    Davies KM, Schwinn KE. Flavonoids in Foods[M]//Andersen OM, Markham KR, eds. Flavonoids: Chemistry, Biochemistry and Applications. Boca Raton: CRC Press Inc., 2006: 143-218.
    [26]
    Hughes J, Hughes MA. Multiple secondary plant product UDP-glucose glucosyltransferase genes expressed in cassava (Manihot esculenta Crantz) cotyledons[J]. Mitochondrial DNA, 1994, 5(1): 41-49.
    [27]
    Sawada S, Suzuki H, Ichimaida F, Yamaguchi M, Iwashita T, FukuiY, Hemmi H, Nishino T, Nakayama T. UDP-glucuronic acid: anthocyanin glucuronosyltransferase from red daisy (Bellis perennis) flowers[J]. J Biol Chem, 2005, 280(2): 899-906.
    [28]
    Gachon CM, Meurinne ML, Saindrenan P. Plant secondary metabolism glycosyltransferases: the emerging functional analysis[J]. Trends Plant Sci, 2005, 10(11): 542-549.
    [29]
    Sakakibara KY, Hanada K. An evolutionary view of functional diversity in family 1 glycosyltransferases[J].Plant J, 2011, 66(1): 182-193.
    [30]
    Wang XQ. Structure, mechanism and engineering of plant natural product glycosyltransferases[J].FEBS Lett, 2009, 583(20): 3303-3309.
    [31]
    Shao H, He XZ, Achnine L, Blount JW, Dixon RA, Wang XQ. Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula[J]. Plant Cell, 2005, 17(11): 3141-3154.
    [32]
    Li LN, Modolo LV, Trevino LE, Achnine L, Dixon RA, Wang XQ. Crystal structure of Medicago truncatula UGT85H2-insights into the trusctural basis of a multifunctional (iso) flavonoid glycosyltransferase[J]. J Mol Biol, 2007, 370(5): 951-963.
    [33]
    Modolo LV, Li LN, Pan HY, Blount JW, Dixon RA, Wang XQ. Crystal structures of glycosyltransferase UGT78G1 reveal the molecular basis for glycosylation and deglycosylation of (iso) flavonoids[J]. J Mol Biol, 2009, 392(5): 1292-1302.
    [34]
    Kubo A, Arai Y, Nagashima S, Yoshikawa T. Alteration of sugar donor specificities of plant glycosyltransferases by a single point mutation[J].Arch Biochem Biophys, 2004, 429(2): 198-203.
    [35]
    Gong ZZ, Yamazaki M, Sugiyama M, Tanaka Y, Saito K. Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens[J]. Plant Mol Biol,1997, 35(6): 915-927.
    [36]
    黄春辉, 俞波, 苏俊, 舒群, 滕元文. ‘美人酥’和 ‘云红梨1号’红皮砂梨果实的着色生理[J].中国农业科学, 2010, 43(7): 1433-1440.
    [37]
    张琼, 王红清, 冷平, 贾乐新. 草莓果实发育过程中花色苷和黄酮醇类物质的形成机制[J]. 园艺学报, 2008, 35(12): 1735-1741.
    [38]
    王惠聪, 黄旭明, 胡桂兵, 黄辉白. 荔枝果皮花青苷合成与相关酶的关系研究[J]. 中国农业科学, 2004, 37(12): 2028-2032.
    [39]
    Boss PK, Davies C, Robinson SP. Expression of anthocyanin biosynthesis pathway genes in red and white grapes[J]. Plant Mol Biol, 1996, 32(3): 565-569.
    [40]
    De Paoli E, Dorantes-Acosta A, Zhai JX, Accerbi M, Jeong DH, Park S, Meyers BC, Jorgensen RA, Green PJ. Distinct extremely abundant siRNAs associated with cosuppression in petunia[J].RNA, 2009, 15(11): 1965-1970.
    [41]
    唐亚萍, 原慧, 覃建兵. 天山雪莲UDP葡萄糖-类黄酮-3-O-葡萄糖基转移酶基因的克隆及功能分析[J].生物工程学报, 2012, 28(6): 705-714.
    [42]
    Zeng SH, Liu YL, Hu WM, Liu YL, Shen XF, Wang Y. Integrated transcriptional and phytoche-mical analyses of the flavonoid biosynthesis pathway in Epimedium[J]. Plant Cell Tiss Org, 2013, 115(3): 355-365.
    [43]
    Huang WJ, Sun W, Lv HY, Luo M, Zeng SH, Pattanaik S, Yuan L, Wang Y. A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway[J]. PloS One, 2013, 8(8): 1-16.
    • Related Articles

  • Related Articles

    [1]Li Jin-Ye, Ping Jing-Yao, Cui Gui-Feng, Su Ying-Juan, Wang Ting. Effects of the broken rps2 gene cluster on evolutionary rates in Campanulaceae[J]. Plant Science Journal, 2023, 41(3): 333-342. DOI: 10.11913/PSJ.2095-0837.22231
    [2]Wang Jie, Wei Ai-Li, Shi Ying, Li Yan-Hui, Han Yu-Xin, Wang Zhong-Jie. Adaptive evolutionary analysis of hetR gene in Nostoc[J]. Plant Science Journal, 2020, 38(1): 23-31. DOI: 10.11913/PSJ.2095-0837.2020.10023
    [3]Ping Jing-Yao, Zhu Ming, Su Ying-Juan, Wang Ting. Molecular evolution of chloroplast gene rps12 in ferns[J]. Plant Science Journal, 2020, 38(1): 1-9. DOI: 10.11913/PSJ.2095-0837.2020.10001
    [4]Liang Ying-Yi, Pang Xue-Qun, Wang Ting. Mechanism and evolution of fruit type diversity[J]. Plant Science Journal, 2017, 35(6): 912-924. DOI: 10.11913/PSJ.2095-0837.2017.60912
    [5]Wu Xiao-Ping, Sen Lin, Chen Nan, Zhang Xiao, Ma Zhao-Xia, Zhang Qin-Yu. Study on the molecular evolution of the psaA gene from ferns[J]. Plant Science Journal, 2017, 35(2): 177-185. DOI: 10.11913/PSJ.2095-0837.2017.20177
    [6]XU Ke, WANG Bo, SU Ying-Juan, GAO Lei, WANG Ting. Molecular Evolution of psbD Gene in Ferns：Selection Pressure and Co-evolutionary Analysis[J]. Plant Science Journal, 2013, 31(5): 429-438. DOI: 10.3724/SP.J.1142.2013.50429
    [7]ZHOU Yuan, WANG Bo, GAO Lei, WANG Ting. Adaptive Evolution and Coevolution of the rbcL Gene in Xeric Pteridaceae Ferns[J]. Plant Science Journal, 2011, 1(4): 409-416.
    [8]Wang Chongyun, Dang Chenglin. PLANT MATING SYSTEM AND ITS EVOLUTIONARY MECHANISM IN RELATION TO POPULATION ADAPTATION[J]. Plant Science Journal, 1999, 17(2): 163-172.
    [9]Xu Naiyu. THE TAXONOMY,ORIGIN AND EVOLUTION OF WHEAT[J]. Plant Science Journal, 1988, 6(2): 187-194.
    [10]Wang Jinwu, Li Maoxue, Li Lixia. STUDIES ON THE CYTOTAXONOMY OF POLYGONATUM Ⅰ.Karyotypes and evolution of eight species of Polygonatum in China[J]. Plant Science Journal, 1987, 5(1): 1-10.

  • Cited by

    Periodical cited type(7)

    1. 席欧彦，王晨日，古丽奴尔·吐拉西，胡红英. 伊犁河谷野果林传粉昆虫物种多样性及访花行为特征. 新疆农业科学. 2024(01): 190-198 .
    2. 管岳，申文靖，陆彪，王妍欣，阿克居力得孜·努尔改里得，周龙. 塔额盆地野果林不同居群野扁桃土壤种子库及幼苗更新研究. 西北植物学报. 2024(06): 961-967 .
    3. 热依汉古丽·夏迪，杨蕾，如马南木·尼合买提，贾贤德，巫利梅，吕海英. 西天山野果林准噶尔山楂土壤种子库海拔梯度分布格局. 植物科学学报. 2023(02): 172-182 . 本站查看
    4. 李波，赵阳，刘婷，陈学龙，高本强，曹秀文. 洮河上游紫果云杉群落土壤种子库特征及其与地上植被的关系. 西北植物学报. 2022(04): 705-714 .
    5. 冯琳骄，褚佳瑶，孟雨欣，周龙，陆彪. 不同居群天山樱桃土壤种子库与幼苗更新特征. 中南林业科技大学学报. 2022(12): 91-97 .
    6. 尚志福，郑友琪，张济显，位竹君，张北方，张莹. 金花小檗种子总黄酮的优化提取及抗氧化活性. 特产研究. 2021(05): 75-80 .
    7. 陈本学，李雁冰，范少辉，刘广路，申景昕. 海南甘什岭白藤土壤种子库特征及幼苗更新能力. 生态学杂志. 2020(04): 1091-1100 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (1329) PDF downloads (1719) Cited by(11)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return