Advance Search
WANG Yan, GAO Peng, HUANG Min, CHEN Hao, YANG Zhi-Rong, SUN Qun. Effects of High Temperature on the Activity and Expression of Antioxidative Enzymes in Rice Flag Leaves during the Flowering Stage[J]. Plant Science Journal, 2015, 33(3): 355-361. DOI: 10.11913/PSJ.2095-0837.2015.30355
Citation: WANG Yan, GAO Peng, HUANG Min, CHEN Hao, YANG Zhi-Rong, SUN Qun. Effects of High Temperature on the Activity and Expression of Antioxidative Enzymes in Rice Flag Leaves during the Flowering Stage[J]. Plant Science Journal, 2015, 33(3): 355-361. DOI: 10.11913/PSJ.2095-0837.2015.30355
shu

Effects of High Temperature on the Activity and Expression of Antioxidative Enzymes in Rice Flag Leaves during the Flowering Stage

  • 1. College of Life Sciences, Sichuan University, Chengdu 610064, China;

  • 2. Sichuan Institute of Atomic Energy, Chengdu 610066, China

More Information
  • Received Date: October 26, 2014
  • Available Online: October 31, 2022
  • Published Date: June 27, 2015
    Graphical Abstract
    • Abstract
  • We determined the chlorophyll content, activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), as well as the gene expression of SOD, CAT and POD in rice flag leaves during the flowering stage under high temperature stress. We used real-time RT-PCR to disclose the mechanism of the physiological responses and adaptation of ‘Ⅱ YOU 838’ to heat stress. Upon heat treatment, the decline in chlorophyll content and the Chl a/Chl b ratio in ‘Ⅱ YOU 838’ was to a lesser degree than that of ‘Gang YOU 725’, and the activity of SOD and POD increased by a higher degree and the activity of CAT declined by a lower degree during the flowering stage. The expressions of the three antioxidative enzyme genes in ‘Ⅱ YOU 838’ were significantly higher than those in ‘Gang YOU 725’. These results implied that ‘Ⅱ YOU 838’ may have higher tolerance to heat stress by maintaining higher photosynthetic metabolism and improving protective antioxidant activity and expression.
    • FullText(HTML)
    • References (25)
  • [1]
    朱德峰, 程式华, 张玉屏, 林贤青, 陈惠哲. 全球水稻生产现状与制约因素分析[J].中国农业科学, 2010, 43(3): 474-479.
    [2]
    段骅, 杨建昌. 高温对水稻的影响及其机制的研究进展[J]. 中国水稻科学, 2012, 26(4): 393-400.
    [3]
    李飞, 卓壮, U. A. Kapila Siri Udawela, 高用明, 石英尧. 水稻高温热害发生机理与耐高温遗传基础研究[J]. 植物遗传资源学报, 2013, 14(1): 97-103.
    [4]
    邓文敏, 邓达胜, 吴万义, 杨成明, 刘勇强, 杨东情, 陈丽华. 杂交水稻恢复系辐恢838的选育与应用[J]. 辐射研究与辐射工艺学报, 2005, 23(2): 125.
    [5]
    黄智敏, 成尚廉, 李成尧, 邓贻仁, 万守纯, 李祖仑, 张正涛. Ⅱ优838作中稻栽培的结实率与耐高温性状[J]. 湖北气象, 1998(1): 21-23.
    [6]
    雷东阳, 陈立云, 李稳香, 姜琴, 张桂莲. 杂交水稻抽穗扬花期高温对结实率及相关生理特性的影响[J]. 杂交水稻, 2006, 21(3): 68-71.
    [7]
    黄智敏, 成尚廉, 李成尧.杂交中稻Ⅱ优838耐高温性分析[J].杂交水稻, 1998, 13(4):34-35.
    [8]
    王复标, 黄福灯, 程方民, 李兆伟, 胡东维, 潘刚, 毛愉婵. 水稻生育后期叶片早衰突变体的光合特性与叶绿体超微结构观察作物学报[J]. 作物学报, 2012, 38(5): 871-879.
    [9]
    韦金池, 杨海灵. 水稻谷胱甘肽过氧化物酶基因OsGPX3和OsGPX4的分子特性研究[J]. 植物科学学报, 2013, 31(1): 64-72.
    [10]
    Mittal D, Madhyastha DA, Grover A. Gene expression analysis in response to low and high temperature and oxidative stresses in rice: Combination of stresses evokes different transcriptional changes as against stresses applied individually[J]. Plant Sci, 2012, 197: 102-113.
    [11]
    Shah K, Nahakpam S. Heat exposure alters the expression of SOD, POD, APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars[J]. Plant Phy-siol Bioch, 2012, 57: 106-113.
    [12]
    邓达胜, 陈浩, 邓文敏, 冯磊, 杨学锋, 马安禄, 刘明海. 水稻恢复系辐恢838及其衍生系的选育和应用[J]. 核农学报, 2009, 23(2): 175-179.
    [13]
    杨敏文. 快速测定植物叶片叶绿素含量方法的探讨[J]. 光谱实验室, 2002, 19(4): 478-481.
    [14]
    黄敏, 王艳, 潘琳, 杨成明, 孙群, 高鹏, 伍玲, 杜晓颖, 雷庆. 杂交水稻中耐热基因的筛选和验证[J]. 西南农业学报, 2009, 22(5): 1199-1203.
    [15]
    Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Res, 2001, 29(9): 2002-2007.
    [16]
    张桂莲, 陈立云, 张顺堂, 刘国华, 唐文邦, 贺治洲, 王明. 抽穗开花期高温对水稻剑叶理化特性的影响[J]. 中国农业科学, 2007, 40(7): 1345-1352.
    [17]
    杜尧东, 李键陵, 王华, 唐湘如, 胡飞. 高温胁迫对水稻剑叶光合和叶绿素荧光特征的影响[J]. 生态学杂志, 2012, 31(10): 2541-2548.
    [18]
    Ueda Y, Uehara N, Sasaki H, Kobayashi K, Yamakawa T. Impacts of acute ozone stress on superoxide dismutase (SOD) expression and reactive oxygen species (ROS) formation in rice lea-ves[J]. Plant Physiol Bioch, 2013, 70: 396-402.
    [19]
    苏鹏, 冯慧, 陈浩, 杨成明, 吴孝波, 黄强. 杂交水稻在自然高温胁迫下的耐热性研究[J]. 西南农业学报, 2013, 26(4): 1387-1391.
    [20]
    高俊杰, 秦爱国, 于贤昌. 低温胁迫下嫁接对黄瓜叶片SOD和CAT基因表达与活性变化的影响[J]. 应用生态学报, 2009, 20(1): 213-217.
    [21]
    Noctor G, Foyer CH. Ascorbate and glutathione: Keeping active oxygen under control[J]. Ann Rev Plant Physiol Mol Biol, 1998, 49: 249-279.
    [22]
    Morita S, Nakatani S, Koshiba T, Masumura T, Ogihara Y, Tanaka K. Differential expression of two cytosolic ascorbate peroxidases and two superoxide dismutase genes in response to abiotic stress in rice[J]. Rice Sci, 2011, 18(3): 157-166.
    [23]
    李婷, 韩莹琰, 范双喜, 任月. 叶用莴苣荧光定量PCR体系的优化[J]. 中国农学通报, 2013, 29(13): 106-113.
    [24]
    梁国庆, 孙静文, 周卫, 王秀斌. 钙对苹果果实超氧化物歧化酶、过氧化氢酶活性及其基因表达的影响[J]. 植物营养与肥料学报, 2011, 17(2): 438-444.
    [25]
    赵士诚, 孙静文, 马有志, 汪洪, 梁国庆, 周卫. 镉对玉米幼苗活性氧代谢、超氧化物歧化酶和过氧化氢酶活性及其基因表达的影响[J]. 中国农业科学, 2008, 41(10): 3025-3032.
    • Related Articles

  • Related Articles

    [1]Rao Li-Ping, Su Wen-Jin, Liu Yi, Song Tian-Xiao, Soviguidi Deka Reine Judesse, Yang Xin-Sun, Zhang Wen-Ying. Cloning and expression analysis of Ipomoea batatas (L.) Lam. gene IbPAL[J]. Plant Science Journal, 2020, 38(3): 360-368. DOI: 10.11913/PSJ.2095-0837.2020.30360
    [2]Liu Tao, Xiong Qin, Xu Ying-Yan, Cai Wei-Wei, Lü Shi-Heng, She Wen-Qin, Chen Gui-Xin. Selection of reference genes for qRT-PCR normalization in Cestrum nocturnum during flowering[J]. Plant Science Journal, 2017, 35(4): 534-542. DOI: 10.11913/PSJ.2095-0837.2017.40534
    [3]CAO Jing, XU Dong-Sheng, HUANG Dai-Hong, YUAN Jun-Wen, ZHAO Juan, WANG Wen-Yan, LAN Hai-Yan. Cloning, Characterization, and Functional Analysis of Seed Coat Mucilage-related Gene TTG1 from Lepidium perfoliatum[J]. Plant Science Journal, 2014, 32(4): 371-382. DOI: 10.3724/SP.J.1142.2014.40371
    [4]CHEN Ling, SU Pei-Pei, TONG Han-Wen, LIU Yi-Ke, ZHU Zhan-Wang, YANG Guang-Xiao, GAO Chun-Bao, HE Guang-Yuan. Isolation of the Promoter Region of a Pollen-specific Gene PSG076 by Inverse-PCR[J]. Plant Science Journal, 2012, (3): 309-314. DOI: 10.3724/SP.J.1142.2012.30309
    [5]YANG Mei, XU Li-Ming, LIU Yan-Ling. Optimization and Establishment of SRAP-PCR in Lotus[J]. Plant Science Journal, 2012, 30(1): 85-91. DOI: 10.3724/SP.J.1142.2012.10085
    [6]ZHANG Yu-Bao, XIE Zhong-Kui, WANG Ya-Jun, GUO Zhi-Hong, TONG Xun-Zhang. Simultaneously Detection of Two Main Lanzhou Lily (Lilium davidii var. unicolor) Viruses by RT-PCR[J]. Plant Science Journal, 2010, 28(6): 744-749.
    [7]ZHUANG Dong-Hong, HUANG Xiu-Li, WU Yi-Rui, LIN Yi-Han, ZHENG Han-Pan. Optimization and Application of RAPD-PCR System for Cucurbita[J]. Plant Science Journal, 2005, 23(5): 422-426.
    [8]LI Jing-Fang, HUANG Shao-Yi, TIAN Ren-Peng, ZHANG Luo-Zhen, FANG Cheng-Xiang. An Improved Method Suited to Extract Total RNA Used to RT-PCR from Fir Plant[J]. Plant Science Journal, 2004, 22(6): 551-556.
    [9]WANG Li, YI Ping, WAN Cui-Xiang, ZHU Ying-Guo. AP-PCR Analysis for Mitochondria DNA of CMS Rice Honglian Type[J]. Plant Science Journal, 2002, 20(6): 405-408.
    [10]Peng Yanhua, Huang Yongxiu, Qi Yipeng. A COUPLED ONE-STEP METHOD OF REVERSE TRANSCRIPTION(RT)FOR GENERATION OF OPEN READING OF G PROTEIN αSUBUNIT GENE[J]. Plant Science Journal, 1996, 14(2): 97-100.

Catalog

    Get Citation
    PDF
    XML
    Article views (1909) PDF downloads (1906) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return