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YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
Citation: YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
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Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress

  • Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

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  • Received Date: February 15, 2015
  • Published Date: August 27, 2015
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    • Abstract
  • A gene encoded potassium transporter was cloned from Halostachys caspica by SMARTTM RACE, and homology analysis by BLAST showed that it had high identity with AtKUP12 from Arabidopsis thaliana, so named HcKUP12. Results showed that HcKUP12 contained a 262 bp 5'-UTR, 147 bp 3'-UTR and 2544 bp open reading frame encoding 847 amino acids with a molecular weight of 93.31 kD and an isoelectric point of 7.19. The expression profile of HcKUP12 was examined under 600 mmol/L NaCl stress during 24 h using real-time qRT-PCR. Results indicated that its expression level was significantly increased and showed the highest level of expression (225 times higher than that of the control) after 24 h treatment. This suggests that the HcKUP12 gene might be a candidate gene related to salt tolerance in Halostachys caspica.
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    • References (25)
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