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Zhou Si-Yu, Wang Yong-Qing. Relationship between embryonic callus induction from leaves and endogenous hormone content in loquat (Eriobotrya japonica Lindl.)[J]. Plant Science Journal, 2017, 35(1): 99-106. DOI: 10.11913/PSJ.2095-0837.2017.10099
Citation: Zhou Si-Yu, Wang Yong-Qing. Relationship between embryonic callus induction from leaves and endogenous hormone content in loquat (Eriobotrya japonica Lindl.)[J]. Plant Science Journal, 2017, 35(1): 99-106. DOI: 10.11913/PSJ.2095-0837.2017.10099
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Relationship between embryonic callus induction from leaves and endogenous hormone content in loquat (Eriobotrya japonica Lindl.)

  • College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

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This work was supported by a grant from the Sichuan Provincial Science and Technology Support Project(2016NYZ0034).

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  • Received Date: July 12, 2016
  • Available Online: October 31, 2022
  • Published Date: February 27, 2017
    Graphical Abstract
    • Abstract
  • To understand the relationship between endogenous hormone content during embryonic callus induction, somatic embryos were obtained by embryonic callus induction from loquat leaves. The content of four endogenous hormones, gibberellic acid (GA3), auxin (IAA), abscisic acid (ABA) and zeatin (ZT), were analyzed by high performance liquid chromatography (HPLC). The dynamic changes of these endogenous hormones in embryonic calli were then explored. Results suggested that the ratio of IAA/ZT in leaves had a positive effect on embryonic callus induction, whereas GA3/IAA had a negative effect. Low content of GA3 and high content of IAA and ABA were required for embryonic calli induction, and a high ratio of IAA/ZT was advantageous to their formation. The timely addition of exogenous hormones in the late period of subculture was helpful for maintaining the embryo. This research provides a theoretical direction for explant selection, extraneous hormone addition to calli subculture, and successive transfer time selection, as well as a foundation for efficiently obtaining embryonic materials and developing the study of gene transformation.
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    • References (21)
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