Advance Search
Cao Rui, Chen Hao, Ding Yi. Comparison of protein extraction methods and two-dimensional electrophoresis analysis in sacred lotus seeds[J]. Plant Science Journal, 2018, 36(1): 127-135. DOI: 10.11913/PSJ.2095-0837.2018.10127
Citation: Cao Rui, Chen Hao, Ding Yi. Comparison of protein extraction methods and two-dimensional electrophoresis analysis in sacred lotus seeds[J]. Plant Science Journal, 2018, 36(1): 127-135. DOI: 10.11913/PSJ.2095-0837.2018.10127
shu

Comparison of protein extraction methods and two-dimensional electrophoresis analysis in sacred lotus seeds

  • College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China

Funds: 

This work was supported by a grant from the National Natural Science Foundation of China (31271310).

More Information
  • Received Date: October 09, 2017
  • Available Online: October 31, 2022
  • Published Date: February 27, 2018
    Graphical Abstract
    • Abstract
  • Nelumbo nucifera Gaertn. (sacred lotus) is not only an important aquatic vegetable crop, but also good material for basic scientific research. The extraction of sacred lotus seed proteins was optimized using four protein extraction methods (new TCA/acetone, traditional TCA/acetone, improved Tris-HCl, and Tris-saturated phenol methods) combined with two-dimensional electrophoresis (2-DE) technology. The 2-DE analysis showed that the resulting protein profile/map exhibited consistency with the composition and distribution of the sacred lotus seed proteome in ‘Tielian’. Analysis by PDQuest software showed that the new TCA/acetone method was suitable for 2-DE protein extraction of lotus cotyledons and plantules, whereas the traditional TCA/acetone method was suitable for the 2-DE protein extraction of hypocotyls. This work lays a foundation for further research on the sacred lotus seed proteome by mass spectrometry.
    • FullText(HTML)
    • References (20)
  • [1]
    梁宇,荆玉祥,沈世华. 植物蛋白质组学研究进展[J]. 植物生态学报, 2004, 28(1):114-125.

    Liang Y, Jing YX, Shen SH. Advances in plant proteomics[J]. Acta Phytoecologica Sinica, 2004, 28(1):114-125.
    [2]
    Ming R, VanBuren R, Liu YL, Yang M, Han YP, Li LT,et al. Genome of the long-living sacred lotus (Nelumbo nucifera Gaertn.)[J]. Genome Biol, 2013, 14(5):R41.
    [3]
    曾广娟,李春敏,张新忠,陈东玫,赵永波,董文轩. 适于SDS-PAGE分析的苹果叶片蛋白质提取方法[J]. 华北农学报, 2009, 24(2):75-78.

    Zeng GJ, Li CM, Zhang XZ, Chen DM, Zhao YB, Dong WX. Leaf protein extraction methods suitable for SDS-PAGE analysis in apple[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(2):75-78.
    [4]
    陈晶瑜,郭宝峰,何付丽,曲春鹤,尹克鑫,杨洪达,赵长山. 适合双向电泳的植物全蛋白提取方法比较[J]. 中国农学通报, 2010, 26(23):97-100.

    Chen JY, Guo BF, He FL, Qu CH, Yin KX, Yang HD, Zhao CS. The comparison of protein extraction methods of plant for two-dimensional electrophoresis[J]. Chinese Agricultural Science Bulletin, 2010, 26(23):97-100.
    [5]
    Wang W, Vignani R, Scali M, Cresti M. A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis[J]. Electrophoresis, 2006, 27(13):2782-2786.
    [6]
    Moro CF, Fukao Y, Shibato J, Rakwal R,Timperio AM, Zolla L, et al. Unraveling the seed endosperm proteome of the lotus (Nelumbo nucifera Gaertn.) utilizing 1DE and 2DE separation in conjunction with tandem mass spectrometry[J]. Proteomics, 2015, 15(10):1717-1735.
    [7]
    冯海燕,景志忠,房永祥,莫斯科. 双向凝胶电泳技术及其应用[J]. 生物技术通报,2009(1):59-63.

    Feng HY, Jing ZZ, Fang YX, Mo SK. Two-dimensional electrophoresis and its application[J]. Biotechnology Bulletin, 2009(1):59-63.
    [8]
    Wang W, Scali M, Vignani R, Spadafora A, Sensi E, Mazzuca S, et al. Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds[J]. Electrophoresis, 2003, 24(14):2369-2375.
    [9]
    张彩霞,李壮,张利义,田义,康国栋,陈莹,丛佩华. 苹果叶片总蛋白提取及其双向电泳分析[J]. 果树学报, 2012, 29(3):488-492.

    Zhang CX, Li Z, Zhang LY, Tian Y, Kang GD, Chen Y, Cong PH. Extraction and two-dimensional gel electrophoresis analysis of total protein from apple leaves[J]. Journal of Fruit Science, 2012, 29(3):488-492.
    [10]
    张丽军,谢锦云,张健,梁宋平. 2DE中IPG胶条转移到SDS-PAGE中的技术改进[J]. 生命科学研究, 2004, 8(3):225-230.

    Zhang LJ, Xie JY, Zhang J, Liang SP. Improvement of the process of IPG transfer to SDS-PAGE in 2DE[J]. Life Science Research, 2004, 8(3):225-230.
    [11]
    Jiang L, He L, Fountoulakis M. Comparison of protein precipitation methods for sample preparation prior to proteomic analysis[J]. J Chromatogr A, 2004, 1023(2):317-320.
    [12]
    贾宇峰,林秋霞,郭鹞,刘少君. 蛋白质双向电泳图像分析[J].生物化学与生物物理进展, 2001, 28(2):246-250.

    Jia YF, Lin QX, Guo Y, Liu SJ. The image analysis of two-dimensional gel electrophoresis[J]. Progress in Biochemistry and Biophysics, 2001, 28(2):246-250.
    [13]
    赵书宇,刘海英,沈鹏,金正勋,张忠臣,张海彬,王露露. 水稻胚乳双向电泳技术体系的优化分析[J]. 东北农业大学学报, 2012, 43(4):62-66.

    Zhao SY, Liu HY, Shen P, Jin ZX, Zhang ZC, Zhang HB, Wang LL. Optimization of two-dimensional gel electrophoresis system of rice endosperm[J]. Journal of Northeast Agricultural University, 2012, 43(4):62-66.
    [14]
    王发祥,贺伟,李微,俞健,李向红,王建辉,刘永乐. 干莲子及其磨皮粉中蛋白质的组成特性比较[J]. 食品科学, 2015, 36(1):59-62.

    Wang FX, He W, Li W, Yu J, Li XG, Wang JH, Liu YL. Comparison of composition characteristics of proteins in lotus seed with its peel waste[J]. Food Science, 2015, 36(1):59-62.
    [15]
    康俊梅,熊军波,孙彦,李燕,沈静,杨青川. 利用双向电泳技术分离野牛草叶片蛋白的方法研究[J]. 西北农业学报, 2010, 19(2):29-34.

    Kang JM, Xiong JB, Sun Y, Li Y, Shen J, Yang QC. Key technology of separation of Buffalograss leaf proteome by applying two dimensional gel electrophoresis[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2010, 19(2):29-34.
    [16]
    Li WM, Wei ZW, Qiao ZH, Wu ZN, Cheng LX, Wang YY. Proteomics analysis of alfalfa response to heat stress[J]. PLoS One, 2013, 8(12):e82725.
    [17]
    阮松林,马华升,王世恒,忻雅,钱丽华,童建新,等. 植物蛋白质组学研究进展Ⅱ.蛋白质组技术在植物生物学研究中的应用[J]. 遗传, 2006, 28(12):1472-1486.

    Ruan SL, Ma HS, Wang SH, Xin Y, Qian LH, Tong JX, et al. Advances in plant proteomicsⅡ. Application of proteome techniques to plant biology research[J]. Hereditas (Beijing), 2006, 28(11):1472-1486.
    [18]
    Vieille C, Zeikus GJ. Hyperthermophilic enzymes:sources, uses, and molecular mechanisms for thermostability[J]. Microbiol Mol Biol R, 2001, 65(1):1-43.
    [19]
    Shen-Miller J, Lindner P, Xie YM, Villa S, Wooding K, Clarke SG, et al. Thermal-stable proteins of fruit of long-living sacred lotus Nelumbo nucifera Gaertn var. China antique[J]. Trop Plant Biol, 2013, 6(2-3):69-84.
    [20]
    Shen-Miller J. Sacred lotus, the long-living fruits of China antique[J]. Seed Sci Res, 2002, 12(3):131-143.
    • Related Articles

  • Related Articles

    [1]LI Xin-Wei, LI Jian-Qiang, WANG Ying-Ming, WANG Heng-Chang, LI Xiao-Dong. Studies on the Floristic Characteristics and Geographical Distribution of the Genus Clematis Linnaeus from Hubei, China[J]. Plant Science Journal, 2004, 22(4): 294-300.
    [2]ZHENG Gui-Ling, LIU Sheng-Xiang, CHEN Gui-Ying, WANG Chang-Li, CHEN Niu. Studies on the Vertical Distribution of Mosses in Mt. Jiugongshan,Hubei,China[J]. Plant Science Journal, 2002, 20(6): 429-432.
    [3]LIU Song-Bai, LI Jian-Qiang, WANG Ying-Ming, WANG Heng-Chang, LI Xiao-Dong. Some New Recorded Plants of Dryopteris Adanson from Hubei[J]. Plant Science Journal, 2002, 20(6): 425-426.
    [4]Zhang Yinhua, Xiong Xiufang, Xu Ying. ANALYSIS OF HUBEI VOLATILE OIL OF GARDENIA FLOWER BY GC/MS[J]. Plant Science Journal, 1999, 17(1): 61-64.
    [5]Li Jianqiang, Zhang xinhua. A REVISION OF FAGACEAE FROM HUBEI[J]. Plant Science Journal, 1998, 16(3): 241-252.
    [6]Wang Shiyun, Xu Huizhu, Zhao Zien, Peng Fusong, Wan Caigan. A STUDY ON THE CONSERVATION OF THE RARE AND THREATENED PLANTS IN HUBEI AND ITS SURROUNDINGS[J]. Plant Science Journal, 1995, 13(4): 354-368.
    [7]Zheng Jiehua. RESEARCH ON THE BASIC ELEMENTS AND MAIN FEATURES OF PTERIDOPHYTES FLORA IN HUBEI PROVINCE[J]. Plant Science Journal, 1987, 5(3): 227-233.
    [8]Zheng Zhong. ON THE PRECIOUS AND RARE PLANTS IN HUBEI[J]. Plant Science Journal, 1986, 4(3): 279-296.
    [9]Wang Yingming. ON THE VEGETATION REGIONALIZATION OF HUBEI PROVINCE[J]. Plant Science Journal, 1985, 3(2): 165-176.
    [10]Lu Debing, Bi Liejue. A PRELIMINARY REPORT OF CHARACEAE FROM HUBEI AND JIANGXI PROVINCES[J]. Plant Science Journal, 1985, 3(2): 147-155.

  • Cited by

    Periodical cited type(7)

    1. 鲁思勤，段枫，张莉雪，宁国贵，何燕红. 高温胁迫对‘索邦’百合花色合成的影响. 华中农业大学学报. 2024(01): 149-156 .
    2. 刘筱琳，孙婷婷，杨捷，何恒斌. 天香百合、药百合黄酮醇合成酶FLS基因克隆和表达分析. 浙江农业学报. 2024(02): 344-357 .
    3. 王莹，李娟，孙雪梅. 菊芋HtMYB2基因VIGS体系构建与功能验证. 中国农学通报. 2024(24): 116-121 .
    4. 李戌彦，杨忠义，纪薇，杨明霞. 基于不同颜色山楂花MYB家族的挖掘与比较分析. 核农学报. 2021(01): 49-59 .
    5. 毕蒙蒙，曹雨薇，宋蒙，唐玉超，何国仁，杨悦，杨盼盼，徐雷锋，明军. 百合花色研究进展. 园艺学报. 2021(10): 2073-2086 .
    6. 陈露，李家明，周相助，许茹，林义章，钟凤林. 外施苹果酸对紫色小白菜花青苷合成关键基因的影响. 农业生物技术学报. 2020(03): 407-419 .
    7. 段真珍，黄振，周宇明，李慧雪，涂万富，张木清，姚伟. 花青素合成转录因子PAP2植物表达载体构建及其遗传转化. 安徽农业科学. 2020(13): 106-110 .

    Other cited types(3)

Catalog

    Get Citation
    PDF
    XML
    Article views (758) PDF downloads (904) Cited by(10)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return