Advance Search
Zhang Cheng-Lei, Zhou Rui, Xie Shan-Peng, Xie Kai-Dong, Xia Qiang-Ming, Wu Xiao-Meng, Guo Wen-Wu. Exploration and evaluation of morphological traits and primary metabolites of tetraploid seedlings from Hongkong kumquat (Fortunella hindsii Swingle)[J]. Plant Science Journal, 2022, 40(1): 47-53. DOI: 10.11913/PSJ.2095-0837.2022.10047
Citation: Zhang Cheng-Lei, Zhou Rui, Xie Shan-Peng, Xie Kai-Dong, Xia Qiang-Ming, Wu Xiao-Meng, Guo Wen-Wu. Exploration and evaluation of morphological traits and primary metabolites of tetraploid seedlings from Hongkong kumquat (Fortunella hindsii Swingle)[J]. Plant Science Journal, 2022, 40(1): 47-53. DOI: 10.11913/PSJ.2095-0837.2022.10047

Exploration and evaluation of morphological traits and primary metabolites of tetraploid seedlings from Hongkong kumquat (Fortunella hindsii Swingle)

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (32172525),Science and Technology Innovation Strategy Special Fund of Guangdong Province (2018B020202009), and Key Research and Development Program of Hubei Province (2020BBA036, 2021DFE016).

More Information
  • Received Date: September 12, 2021
  • Revised Date: November 09, 2021
  • Available Online: October 31, 2022
  • Published Date: February 27, 2022
  • To explore tetraploid seedlings from polyembryonic Hongkong kumquat (Fortunella hindsii Swingle), we screened eight putative tetraploids (doubled diploids) from 1289 seedlings based on root, leaf, and oil gland morphology. Flow cytometry and molecular identification showed that these tetraploids were all autotetraploids, with a screening accuracy of 100% and natural occurrence rate of 0.62%. Compared with diploids, the tetraploids of Hongkong kumquat showed significantly lower plant height, stem diameter, internode number, internode length, and stomatal density, but higher leaf thickness and stomatal size. In addition, leaf primary metabolites between the tetraploids and corresponding diploids were analyzed by gas chromatography-mass spectrometry (GC-MS). Among the 24 detected primary metabolites, the content of quininic acid in the tetraploid leaves was significantly higher than that in the diploid leaves, while the contents of inositol, 4-aminobutyric acid, and 1-monopalmitin were significantly lower.
  • [1]
    邓秀新. 中国柑橘品种[M]. 北京:中国农业出版社, 2008.
    [2]
    郭文武, 叶俊丽, 邓秀新. 新中国果树科学研究70年——柑橘[J]. 果树学报, 2019, 36(10):1264-1272.

    Guo WW, Ye JL, Deng XX. Fruit scientific research in New China in the past 70 years:Citrus[J]. Journal of Fruit Science, 2019, 36(10):1264-1272.
    [3]
    Zhu CQ, Zheng XJ, Huang Y, Ye JL, Chen P, et al. Genome sequencing and CRISPR/Cas9 gene editing of an early flowering Mini-Citrus (Fortunella hindsii)[J]. Plant Biotechnol J, 2019, 17:2199-2210.
    [4]
    周锐, 解凯东, 王伟, 彭珺, 谢善鹏, 等. 依据多倍体形态特征快速高效发掘柑橘四倍体[J]. 园艺学报, 2020, 47(12):2451-2458.

    Zhou R, Xie KD, Wang W, Peng J, Xie SP, et al. Efficient identification of tetraploid plants from seedling populations of apomictic citrus genotypes based on morphological characteristics[J]. Acta Horticulturae Sinica, 2020, 47(12):2451-2458.
    [5]
    张斯淇, 徐强, 邓秀新. 无融合生殖与柑橘多胚现象的研究进展[J]. 植物科学学报, 2014, 32(1):88-96.

    Zhang SQ, Xu Q, Deng XX. Advances in apomixis and polyembryony research in Citrus plants[J]. Plant Science Journal, 2014, 32(1):88-96.
    [6]
    梁武军, 解凯东, 郭大勇, 谢宗周, 伊华林, 郭文武. 10个柑橘砧木类型同源四倍体的发掘与SSR鉴定[J]. 果树学报, 2014, 31(1):1-6.

    Liang WJ, Xie KD, Guo DY, Xie ZZ, Yi HL, Guo WW. Spontaneous generation and SSR molecular characterization of autotetraploids in ten citrus rootstocks[J]. Journal of Fruit Science, 2014, 31(1):1-6.
    [7]
    彭滢, 李晓妍, 肖璇. 柑橘多胚性砧木枳橙同源四倍体的发掘与SSR鉴定[J]. 分子植物育种, 2020, 18(4):1211-1215.

    Peng Y, Li XY, Xiao X. Excavation and SSR identification of autotetraploids in citrus polyembryonic rootstock citrange[J]. Molecular Plant Breeding, 2020, 18(4):1211-1215.
    [8]
    蒋景龙, 阳妮, 李丽, 秦公伟, 邓家锐, 等. 衢州香橙四倍体种质发掘及形态特征性评价[J]. 果树学报, 2021, 38(5):655-663.

    Jiang JL, Yang N, Li L, Qin GW, Deng JR, et al. Identification and characterization of tetraploids from seedlings of Citrus junos ‘Quzhou xiangcheng’[J]. Journal of Fruit Science, 2021, 38(5):655-663.
    [9]
    梁武军, 解凯东, 郭大勇, 谢宗周, 徐强, 等. 柑橘10个品种实生后代多倍体的发掘及SSR鉴定[J]. 园艺学报, 2014, 41(3):409-416.

    Liang WJ, Xie KD, Guo DY, Xie ZZ, Xu Q, et al. Spontaneous generation and SSR characterization of polyploids from ten citrus cultivars[J]. Acta Horticulturae Sinica, 2014, 41(3):409-416.
    [10]
    解凯东, 彭珺, 袁东亚, 强瑞瑞, 谢善鹏, 等. 以本地早橘和槾橘为母本倍性杂交创制柑橘三倍体[J]. 中国农业科学, 2020, 53(23):4961-4968.

    Xie KD, Peng J, Yuan DY, Qiang RR, Xie SP, et al. Production of citrus triploids based on interploidy crossing with Bendizao and Man tangerines as female parents[J]. Scientia Agricultura Sinica, 2020, 53(23):4961-4968.
    [11]
    夏强明. 基于2n雌配子有性群体定位柑橘着丝粒及其序列特征分析[D]. 武汉:华中农业大学, 2020:23.
    [12]
    Cheng YJ, Guo WW, Yi HL, Pang XM, Deng XX. An efficient protocol for genomic DNA extraction from Citrus species[J]. Plant Mol Biol Rep, 2003, 21(2):177-178.
    [13]
    Xu Q, Chen LL, Ruan XA, Chen DJ, Zhu AD, et al. The draft genome of sweet orange (Citrus sinensis)[J]. Nat Genet, 2013, 45(1):59-66.
    [14]
    Luro FL, Costantino G, Terol J, Argout X, Allario T, et al. Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping[J]. BMC Genomics, 2008, 9:287.
    [15]
    García-lor A, Luro F, Navarro L, Ollitrault P. Comparative use of InDel and SSR markers in deciphering the interspecific structure of cultivated citrus genetic diversity:a perspective for genetic association studies[J]. Mol Genet Genomics, 2012, 287(1):77-94.
    [16]
    Aleza P, Froelicher Y, Schwarz S, Agustí M, Hernández M, et al. Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment[J]. Ann Bot, 2011, 108(1):37-50.
    [17]
    Cuenca J, Froelicher Y, Aleza P, Juárez J, Navarro L, Ollitrault P. Multilocus half-tetrad analysis and centromere mapping in citrus:evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune’[J]. Heredity, 2011, 107(5):462-470.
    [18]
    宋鑫, 谭丰全, 张苗, 蔡元康, 郭大勇, 等. ‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析[J]. 园艺学报, 2019, 46(1):37-46. Song X, Tan FQ, Zhang M, Cai YK, Guo DY, et al. Metabolic characteristics of interspecific allotetraploid somatic hybrid between ‘Newhall’ navel orange and ‘Eureka’ lemon[J]. Acta Horticulturae Sinica, 2019, 46(1):3746.
    [19]
    Neilson EH, Goodger JQ, Woodrow IE, Moller BL. Plant chemical defense:at what cost?[J]. Trends Plant Sci, 2013, 18(5):250-258.
    [20]
    Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, et al. Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement[J]. BMC Plant Biol, 2015, 15(1):89.
  • Related Articles

    [1]Wang Jun-Jie, Cheng Yue-Hong, Ding Shi-Xiong, Wang Yan, Peng Shuai, Yang Jia-Xin, Sun Hong-Ou, Leng Zhi-Cheng, Hu Guang-Wan. Six new records of Orchidaceae in Sichuan Province[J]. Plant Science Journal, 2021, 39(3): 223-228. DOI: 10.11913/PSJ.2095-0837.2021.30223
    [2]DENG Wei-Qiong, SUN Rong, LI Xiu-Ming, LU Ding, YANG Quan, LU Ke-Yu. Flora Study of Riparian Plants on the Mountain River Banks of the Jiulongjiang River Headstream[J]. Plant Science Journal, 2013, 31(5): 467-476. DOI: 10.3724/SP.J.1142.2013.50467
    [3]WU Ming-Kai, ZHANG Xiao-Ping, CAO Tong. Moss Flora of Huangshan Mountain in Anhui Province,China[J]. Plant Science Journal, 2010, 28(3): 365-375. DOI: 10.3724/SP.J.1142.2010.30365
    [4]ZHENG Ji-Qing, CHEN Tong-Tong, LIU Ying, TANG Gui-Ying, LI Fa-Zeng. Study on the Flora of Culai Mountain in Shandong Province[J]. Plant Science Journal, 2006, 24(1): 27-30.
    [5]WANG Shi-Quan, JIA Ze-Feng, LI Fa-Zeng . Studies on Flora of Laoshan Mountain in Shandong Province[J]. Plant Science Journal, 2001, 19(6): 467-474.
    [6]Song Baohua, Li Fazeng. STUDIES ON FLORA OF KUNYU MOUNTAIN IN SHANDONG PROVINCE[J]. Plant Science Journal, 1999, 17(3): 220-226.
    [7]Zhao Zuocheng, Yang Yichuan, Yang Fuming, Chenglin, Borje Exstam. A STUDY ON THE FLORA OF PEATERY SWAMP PLANTS IN HONGYUAN,SICHUAN,CHINA[J]. Plant Science Journal, 1995, 13(1): 30-36.
    [8]Zang Dekui, Liu Yufeng, Qi Aishou, Du Mingyun. STUDIES ON THE SEED PLANTS FLORA OF TALSHAN MOUNTAIN IN SHANDONG PROVINCE[J]. Plant Science Journal, 1994, 12(3): 233-239.
    [9]Liu Bin. A STUDY ON THE FLORA OF TUOJIAN MOUNTAIN AREA OF THE DABIE MOUNTAINS, ANHUI PROVINCE[J]. Plant Science Journal, 1991, 9(3): 239-246.
    [10]Liu Dengyi. A PRELIMINARY EXPLORATION OF THE FLORA OF THE LIUGUJIAN MOUNTAINOUS REGIONS ON THE BOUNDARY BETWEEN ANHUI AND JIANGXI[J]. Plant Science Journal, 1988, 6(4): 365-370.
  • Cited by

    Periodical cited type(8)

    1. 韦海航,胡力文,李进华,蔡诚,夏家朗,田红灯,覃振凯,刘秀. 红树植物移植后生理适应性研究. 广西林业科学. 2025(01): 73-77 .
    2. 郭璐瑶,苗灵凤,李大东,向丽珊,杨帆. 施氮和增温对降香黄檀幼苗生长发育和生理特征的影响. 植物科学学报. 2022(02): 259-268 . 本站查看
    3. 刘铎,白爽,齐学斌,宁东峰,梁志杰,郭魏,李平. 小麦百农矮抗58幼苗对Na_2SO_4胁迫响应及阈值鉴定. 福建农业学报. 2021(01): 9-16 .
    4. 于霞霞,郭晔红,杜弢,贾存勤,李欠,马兴东,马旭东. 肉苁蓉寄生对梭梭光合特性的影响. 中成药. 2021(09): 2570-2576 .
    5. 徐倩,郭尚敬,魏慧恬,苏艳艳,马青平,冀芦沙. 外源BABA对NaCl胁迫下二月兰幼苗生长和生理特性的影响. 北方园艺. 2020(12): 75-81 .
    6. 马鹏图,苏世平,李毅,种培芳,后有丽,魏斌. 外源脯氨酸对自然干旱下白刺叶片渗透调节与抗氧化酶活性的影响. 甘肃农业大学学报. 2020(04): 121-127+136 .
    7. 杨俊鸾. NaCl胁迫对银杏叶绿素和保护酶活性的影响. 山东林业科技. 2019(03): 49-52 .
    8. 郭晓霄,王小华,侯爱民. 不同氮磷钾施用量对连翘生理特性的影响. 山东林业科技. 2019(04): 71-74 .

    Other cited types(11)

Catalog

    Article views (357) PDF downloads (229) Cited by(19)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return