Establishment and Optimization of High-frequency Shoot Regeneration System of Ligusticum chuanxiong Hort.

We explored optimized induction and differentiation medium and culture conditions to establish a protocol of rapid clonal propagation of Ligusticum chuanxiong Hort.The petioles were the best explant and Murashige and Skoog (MS) medium containing 2.0 mg/L KT and 0.5 mg/L IAA was suitable for high-frequency shoot regeneration.The results suggested that callus were induced from petioles of aseptic seedlings in dark conditions.And then these callus were subcultured for 15 days in light conditions,about 44.4% frequency of shoot regeneration was induced.The rooting percentage of the regenerated shoots reached 90% on 1/2 MS supplemented with 0.5 mg/L NAA and 0.5 mg/L IBA.Approximately 95% rooted shoots survived in a greenhouse.