Isolated Microspore Culture and Plant Regeneration in Purple Flowering Stalk (Brassica compestris ssp. chinensis var. pupurea Hort.)

The influences of the genotype and the activated charcoal were investigated on the embyroid yields of microspore in purple flowering stalk(Brassica compestris ssp. chinensis var. pupurea Hort.)among 5 genotypes. The results showed, the highest mean yield was up to 42 embryoids per petri dish, while the lowest was zero. The efficiency of embryoid increased by 3-fold when the activated charcoal was added into the liquid media properly. The embryoids produced from microspore culture were further conducted to regenerate plantlet. The regeneration frequency was the highest (up to 50.1%) while solidified with 1.2% of agar in the medium. When treated at 4℃ for 10 days, the regeneration frequencies of the embryoids rose from 45% to 65%.The optimal ages of embryoids (20-24 d) were vital to plant regeneration, the regeneration frequency decreased remarkably along with the increase of the age of embryoids. The media, B5 and MS, had no distinct difference for plant regeneration.