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WU Hao, LU Xiao-Cong, WANG You-Wei. The Preliminary Studies on Isolation and Immunity of Ramulus Mori Polysaccharide[J]. Plant Science Journal, 2005, 23(1): 81-84.
Citation: WU Hao, LU Xiao-Cong, WANG You-Wei. The Preliminary Studies on Isolation and Immunity of Ramulus Mori Polysaccharide[J]. Plant Science Journal, 2005, 23(1): 81-84.
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The Preliminary Studies on Isolation and Immunity of Ramulus Mori Polysaccharide

  • 1.

    Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China

  • 2.

    Puyang Hotway Pharmaceuticals Co., Ltd., Puyang, Henan 457000, China

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  • Received Date: April 01, 2004
  • Revised Date: April 28, 2004
  • Published Date: February 27, 2005
    Graphical Abstract
    • Abstract
  • Polysaccharide was isolated and purified from Chinese medicinal herb Ramulus mori (Sangzhi), and determination method for Ramulus mori polysaccharide (RMPS) was developed in this study. Carbon clearance test and dinitrylfluorobenzene-induced delayed type hypersensitivity test (DTH) were used to evaluate effects of RMPS on immune responses of mice treated with dexamethasone. The results indicated that using modified phenol-sulfuric acid method, the content of polysaccharide was 5.5% in Ramulus mori, and 56.6% in RMPS, which exceeded 70% in RMPS after purifying for a few times. The RMPS can improved phagocytic indexα, strengthened phagocytosing function of reticulate endothelial cell and the intensity of delayed type hypersensitivity (DTH) reaction, and enhanced the activity of T cells of the mice treated with dexamethasone, significantly.
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