Transformation of Lettuce with FMDV Epitopes Fused Gene Mediated by Agrobacterium

Plant-derived vaccine is becoming one focus of plant gene engineering,which has advantages such as easy production,low cost,convenient and safe use.In this experiment,the fused gene O₂₁-O₁₄-A₂₁ containing both type O and A FMDV epitopes was introduced into lettuce mediated by agrobacterium.The hygromycin-resistant lettuce calli were obtained by screening on the medium containing antibiotic.Fifty six hygromycin-resistant lettuce seedlings were regenerated.The results of PCR and PCR-Southern blotting indicated that the fusion gene were successfully integrated into the genomes some of transformed lettuce plants.Expression of O₂₁-O₁₄-A₂₁ gene in transformed lettuce plants was proved by RT-PCR(analyses).