Functional Deletion Analysis of <em>FaChit1</em> Promoter Region from <em>Festuca arundinacea</em>

WANG Shi-Ying; WANG Hao-Ru; WANG Jian

doi:10.3724/SP.J.1142.2013.60562

Plant Science Journal > 2013 > 31(6): 562-569. > DOI: 10.3724/SP.J.1142.2013.60562 CSTR: 32231.14.SP.J.1142.2013.60562

WANG Shi-Ying, WANG Hao-Ru, WANG Jian. Functional Deletion Analysis of FaChit1 Promoter Region from Festuca arundinacea[J]. Plant Science Journal, 2013, 31(6): 562-569. DOI: 10.3724/SP.J.1142.2013.60562

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Functional Deletion Analysis of FaChit1 Promoter Region from Festuca arundinacea

1.
Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China
2. College of Agriculture and Life Science, Ankang University, Ankang, Shaanxi 725000, China

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Received Date: April 23, 2013
Revised Date: October 07, 2013
Published Date: December 29, 2013

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Abstract

Abstract

Activation of different promoter fragments by fungal elicitors,dehydration,mechanical wounding,and ethylene were analyzed in transgenic tobacco using transcriptional fusions of FaChit1 5' upstream sequences to the GUS reporter gene (promoter-GUS expression vectors were designated as pFaChit1P-Ⅰ,pFaChit1P-Ⅱ and pFaChit1P-Ⅲ,respectively).Analysis of promoter deletion showed that the FaChit1 promoter conferred the strongest induction of GUS activity in response to treatment with fungal elicitors,but the least induction in response to mechanical wounding;GUS activity could be induced in response to four stress treatments in leaves containing the 651 bp upstream of the FaChit1 start codon,and the fragment between 935 bp and 233 bp upstream of the FaChit1 start codon was sufficient to direct gene expression in response to fungal elicitors,ethylene,dehydration,and mechanical wounding.Results indicated that the FaChit1 promoter was a multiple stress inducible promoter.
- Tall fescue (Festuca arundinacea),
- FaChit1 gene,
- Promoter deletion analysis

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Functional Deletion Analysis of FaChit1 Promoter Region from Festuca arundinacea

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