Responses of the Antioxidant System in <em>Nelumbo nucifera</em> Seedlings to Arsenic Toxicity

FAN Xiang-Rong; YIN Li-Yan; LI Wei; CHANG Feng-Yi

doi:10.3724/SP.J.1142.2013.60570

Plant Science Journal > 2013 > 31(6): 570-575. > DOI: 10.3724/SP.J.1142.2013.60570 CSTR: 32231.14.SP.J.1142.2013.60570

FAN Xiang-Rong, YIN Li-Yan, LI Wei, CHANG Feng-Yi. Responses of the Antioxidant System in Nelumbo nucifera Seedlings to Arsenic Toxicity[J]. Plant Science Journal, 2013, 31(6): 570-575. DOI: 10.3724/SP.J.1142.2013.60570

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Responses of the Antioxidant System in Nelumbo nucifera Seedlings to Arsenic Toxicity

FAN Xiang-Rong^1,2,3,
YIN Li-Yan^1,2,
LI Wei^1,2, ,,
CHANG Feng-Yi^1,2, ,

1.
Key Laboratory of Aquatic Botany and Watershed Ecology, Chinese Academy of Sciences, Wuhan 430074, China
2. Laboratory of Aquatic Plant Biology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
3. University of Chinese Academy of Sciences, Beijing 100049, China

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Received Date: May 23, 2013
Revised Date: October 06, 2013
Published Date: December 29, 2013

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Abstract

Abstract

To study the antioxidant system of Nelumbo nucifera seedlings in response to arsenic stress, compared the influence of two valences of inorganic arsenic, As (Ⅲ) and As (V), on soluble protein content, malondialdehyde(MDA)and the antioxidant system. Results showed that: MDA and soluble protein content of N. nucifera seedlings increased and then decreased with increasing concentration; MDA and protein content were more sensitive to As(Ⅲ)than to As(V)treatment. The Superoxide Dismutase(SOD)was the most sensitive to arsenic treatment; when the concentration of As (Ⅲ)was 2.5 μmol/L and As(V)was 100 μmol/L, the activity of SOD increased significantly. When the concentration of As(Ⅲ) was 10 μmol/L, the activity of Peroxidase(POD)increased significantly. The Catalase(CAT)was more sensitive to As(V). From the experimental results, with the increase in arsenic concentration, the enzyme activities of SOD, CAT and POD increased, which can be used to eliminate reactive oxygen species(ROS) and maintain cell metabolism stability. This study provides some basic data for further study of N. nucifera physiology and growth change, cultivation and transplants with arsenic stress.
- Nelumbo nucifera,
- Antioxidant systems,
- Arsenic

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