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XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
Citation: XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
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Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods

  • 1.

    College of Agriculture, South China Agriculture University, Guangzhou 510642, China

  • 2. School of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen 518055, China

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  • Received Date: January 06, 2013
  • Revised Date: April 19, 2013
  • Published Date: December 29, 2013
    Graphical Abstract
    • Abstract
  • The essential oils of Thymus vulgaris were extracted by steam distillation, organic solvent extraction and supercritical CO2 extraction.The amounts,compositions and relative contents of the essential oils extracted from the three methods were compared.The results showed that the amount of essential oils extracted by steam distillation was 0.21%,the main chemical components were thymol(36.53%), benzene,1-methyl-3-(1-methylethyl)-(14.13%),1,4-cyclohexadiene,1-methyl-4-(1-methylethyl)(8.09%), and caryophyllene(4.14%);the amount of essential oils extracted by organic solvent extraction was 0.19%,the main components were 1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester(55.23%),thymol(8.73%),and 1,4-cyclohexadiene,1-methyl-4-(1-methylethyl)(5.23%);and the amount of essential oils extracted by supercritical CO2 extraction was 0.27%,the main components were thymol(26.68%),2-propenoic acid,3-phenyl-,methyl ester(21.55%), and benzene,1-methyl-3-(1-methylethyl)(9.69%).Based on the quality,major chemical compositions and extraction amount of essential oils,the steam distillation was the best method to extract essential oils from Thymus vulgaris.
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    • References (24)
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