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ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
Citation: ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
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Research on Tissue Culture Technology of Racomitrium japonicum

  • 1.

    College of Life Science Northeast Forestry University, Harbin, 150040, China

  • 2. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China

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  • Received Date: June 08, 2013
  • Revised Date: October 04, 2013
  • Published Date: December 29, 2013
    Graphical Abstract
    • Abstract
  • The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively, and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0.02% HgCl2 for 45-60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added.
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    • References (25)
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